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Inversion of chromatographic elution orders of peptides and its importance for proteomics

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Abstract

Inversion of the order of peptide elution in reversed-phase liquid chromatography under changing separation conditions, such as gradient slope has been considered. Using a six-protein proteolytic peptide standard and available literature data, the occurrence frequency and importance of this phenomenon in proteomic studies utilizing methods of shotgun proteomics and accurate mass and time tags have been evaluated. Feasibility of qualitative and quantitative description of peptide elution order inversion has been demonstrated using a model of critical liquid chromatography. Existing approaches to predict peptide separation and directions of the shifts of chromatographic peaks when the gradient profile changes have been compared.

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Authors and Affiliations

  1. Institute for Energy Problems of Chemical Physics RAS, Leninsky pr. 38, bld. 2, Moscow, 119334, Russia

    I. A. Tarasova, T. Yu. Perlova, M. L. Pridatchenko, A. A. Goloborod’ko, L. I. Levitsky & M. V. Gorshkov

  2. Semenov’s Institute of Chemical Physics, RAS, ul. Kosygina 4, Moscow, 119991, Russia

    V. V. Evreinov & A. V. Gorshkov

  3. CEA, Universite Joseph Fourier, 17 Avenue des Martyrs, Bat. C3, 38054, Grenoble Cedex 9, France

    V. Guryca & C. D. Masselon

  4. INSERM, U880, Grenoble, F-38054, France

    V. Guryca & C. D. Masselon

Authors
  1. I. A. Tarasova
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  2. T. Yu. Perlova
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  3. M. L. Pridatchenko
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  4. A. A. Goloborod’ko
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  5. L. I. Levitsky
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  6. V. V. Evreinov
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  7. V. Guryca
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  8. C. D. Masselon
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  9. A. V. Gorshkov
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  10. M. V. Gorshkov
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Corresponding author

Correspondence to I. A. Tarasova.

Additional information

Published in Russian in Mass-Spektrometria, 2011, Vol. 8, No. 4, pp. 281–292.

The article was translated by the authors.

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Tarasova, I.A., Perlova, T.Y., Pridatchenko, M.L. et al. Inversion of chromatographic elution orders of peptides and its importance for proteomics. J Anal Chem 67, 1014–1025 (2012). https://doi.org/10.1134/S1061934812130102

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  • DOI: https://doi.org/10.1134/S1061934812130102

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