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AFLP analysis of the genetic diversity of closely related rhododendron species of the section rhodorastra (Ericaceae) from Siberia and the Far East of Russia

  • Plant Genetics
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Abstract

Using AFLP analysis, the genetic variation in four population samples of Rhododendron ledebourii, three such samples of Rh. dauricum, two samples of Rh. sichotense, and one sample of Rh. mucronulatum was examined. Application of three selective primer pairs provided genotyping of 87 accessions from the four species at 213 loci. An analysis of molecular variance revealed a high level of differences between all four species (F ST = 0.172). Populations within the species were also differentiated: for Rh. dauricum F ST = 0.245; Rh. ledebourii F ST = 0.264; Rh. sichotense F ST = 0.034. The highest Nei distance was observed for Rh. mucronulatum with Rh. dauricum and Rh. ledebourii (D = 0.057 and 0.069, respectively). Similarity was observed between the species pairs of Rh. ledebourii-Rh. dauricum (0.032) and Rh. sichotense-Rh. mucronulatum (0.034). The Far Eastern species Rh. sichotense and Rh. mucronulatum were characterized by an elevated level of intrapopulation variation. The data from the AFLP-based analysis of differentiation and variation that were thus obtained were compared with the literature data on the morphological criteria that differentiate the species of interest and those regarding the history of the formation of their ranges. The independence of all the species we examined was confirmed.

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Authors and Affiliations

  1. Sukachev Forest Institute, Russian Academy of Sciences, Krasnoyarsk, 660036, Russia

    N. A. Tikhonova

  2. Institute of Plant and Animal Ecology, Russian Academy of Sciences, Yekaterinburg, 620144, Russia

    M. A. Polezhaeva

  3. Abramov Sikhote-Alin State Biosphere Reserve, Primorsky krai, Terney, 692150, Russia

    E. A. Pimenova

Authors
  1. N. A. Tikhonova
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  2. M. A. Polezhaeva
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  3. E. A. Pimenova
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Correspondence to N. A. Tikhonova.

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Original Russian Text © N.A. Tikhonova, M.A. Polezhaeva, E.A. Pimenova, 2012, published in Genetika, 2012, Vol. 48, No. 10, pp. 1153–1161.

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Tikhonova, N.A., Polezhaeva, M.A. & Pimenova, E.A. AFLP analysis of the genetic diversity of closely related rhododendron species of the section rhodorastra (Ericaceae) from Siberia and the Far East of Russia. Russ J Genet 48, 985–992 (2012). https://doi.org/10.1134/S1022795412100110

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