Abstract
The effects of exogenous glucose on the rates of alternative pathways of photosystem II (PSII)-independent electron flow to PSI and of dark respiration in Synechocystis sp. 6803 cells were studied. The presence of glucose was shown to accelerate the electron flow to P700+, the PSI primary electron donor oxidized with Far-red light (FRL), which excites specifically only PSI. An increase in the glucose concentration was accompanied by a further activation of electron flow to PSI, which was supported by the dark donation of reducing equivalents to the electron transport chain. An increase in the external glucose concentration resulted also in the disappearance of lag-phase in the kinetics of P700+ reduction, which was observed in the cells incubated without glucose after FRL switching off. A similarity of nonphotochemical processes of electron transfer to PSI in cyanobacteria and higher plants was supposed, basing on the earlier observed fact of the occurrence of such lagphase in higher plants and its dependence on the exhausting of stromal reductants in the light. Acceleration of dark electron flow to PSI in the presence of glucose, a major respiratory substrate, may indicate the coupling between nonphotochemical processes in the photosynthetic and respiratory chains of electron transport in cyanobacterial cells. A close correlation between photosynthesis and respiration in cyanobacterial cells is also confirmed by a sharp acceleration of respiration with an increase in the glucose concentration in medium.
Similar content being viewed by others
Abbreviations
- FRL:
-
far-red light
- PS:
-
photosystem
- WL:
-
white light
References
Hoefnagel, M.H.N., Atkin, O.K., and Wiskich, J.T., Interdependence between Chloroplasts and Mitochondria in the Light and the Dark, Biochim. Biophys. Acta, 1998, vol. 1366, pp. 235–255.
Kromer, S., Respiration during Photosynthesis, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1995, vol. 46, pp. 45–70.
Kromer, S., Malmberg, G., and Gardestrom, P., Regulation of the Supply of Cytosolic Oxaloacetate for Mitochondrial Metabolism via Phosphoenolpyruvate Carboxylase in Barley Leaf Protoplasts, Biochim. Biophys. Acta, 1996, vol. 1289, pp. 343–350.
Scherer, S., Do Photosynthetic and Respiratory Electron Transport Chains Share Redox Proteins? Trends Biochem. Sci., 1990, vol. 15, pp. 458–462.
Bukhov, N.G. and Carpentier, R., Alternative Photosystem I-Driven Electron Transport Routes: Mechanisms and Functions, Photosynth. Res., 2004, vol. 82, pp. 17–33.
Egorova, E.A. and Bukhov, N.G., Populations of Photosystem 1 Units Rapidly and Slowly Reduced by Stromal Reductants Represent Photosystem 1α and Photosystem 1β Complexes: Evidence from Irradiance-Response Curves of P700 Photooxidation in Intact Barley Leaves, Photosynthetica, 2005, vol. 43, pp. 147–151.
Serebriiskaya, T.S. and Los, V.F., Functional Characterization of the slr1944 Gene of Cyanobacterium Synechocystis sp. PCC 6803, Fiziol. Rast. (Moscow), 2004, vol. 51, pp. 860–871 (Russ. J. Plant Physiol., Engl. Transl., pp. 774–783).
Shoumskaya, M.A., Paithoonrangsarid, K., Kanesaki, Yu., Los, D.A., Zinchenko, V.V., Tanticharoen, M., Suzuki, I., and Murata, N., Identical Hik-Pre Systems Are Involved in Perception and Transduction of Salt Signals and Hyperosmotic Signals but Regulate the Expression of Individual Genes to Different Extents in Synechocystis, J. Biol. Chem., 2005, vol. 280, pp. 21531–21538.
Schreiber, U., Klughammer, C., and Neubauer, C., Measuring P700 Absorbance Changes around 830 nm with a New Type of Pulse Modulated System, Z. Naturforsch., 1988, vol. 43c, pp. 686–698.
Sholts, K.F. and Ostrovskii, D.N., Cell for Amperometric Determination of Oxygen, Metody sovremennoi biokhimii (Methods of Modern Biology), Kretovich, V.L. and Sholts, K.F., Eds., Moscow: Nauka, 1975, pp. 52–58.
Nikolaeva, M.K., Bukhov, N.G., and Egorova, E.A., Activities of Noncyclic and Alternative Pathways of Photosynthetic Electron Transport in Leaves of Broad Bean Plants Grown at Various Light Irradiances, Fiziol. Rast. (Moscow), 2005, vol. 52, pp. 485–491 (Russ. J. Plant Physiol., Engl. Transl., pp. 427–433).
Bukhov, N.G. and Egorova, E.A., Nonmonotonic Redox Conversions of P700 in Leaves Irradiated with Far-Red Light Originate from Variable Contributions of Several Alternative Electron Transport Pathways during the Induction Period, Fiziol. Rast. (Moscow), 2005, vol. 52, pp. 645–652 (Russ. J. Plant Physiol., Engl. Transl., pp. 571–577).
Egorova, E.A., Nikolaeva, M.K., and Bukhov, N.G., Origin of Multiphase Reduction of P700+ in Broad Bean Leaves after Irradiation with Far-Red Light, Fiziol. Rast. (Moscow), 2005, vol. 52, pp. 492–498 (Russ. J. Plant Physiol., Engl. Transl., pp. 434–440).
Bukhov, N.G., Egorova, E.A., and Carpentier, R., Electron Flow to Photosystem I from Stromal Reductants In Vivo: The Size of the Pool of Stromal Reductants Controls the Rate of Electron Donation to Both Rapidly and Slowly Reducing Photosystem I Units, Planta, 2002, vol. 215, pp. 812–820.
Asada, K., Heber, U., and Schreiber, U., Pool Size of Electrons That Can Be Donated to P700+, as Determined in Intact Leaves: Donation to P700+ from Stromal Components via the Intersystem Chain, Plant Cell Physiol., 1992, vol. 33, pp. 927–932.
Egorova, E.A., Bukhov, N.G., Kurkova, E.B., Myasoedov, N.A., Balnokin, Yu.V., and Popova, L.G., Monophasic Kinetics of Electron Donation from Stromal Reductants in Unicellular Halophytic Alga Tetraselmis viridis: Relation of the Function to Characteristic Structure of Chloroplast Membrane System, Fiziol. Rast. (Moscow), 2006, vol. 53, pp. 165–171 (Russ. J. Plant Physiol., Engl. Transl., pp. 147–152).
Egorova, E.A., Bukhov, N.G., Heber, U., Samson, G., and Carpentier, R., Effect of the Pool Size of Stromal Reductants on the Alternative Pathway of Electron Transfer to Photosystem I in Chloroplasts of Intact Leaves, Fiziol. Rast. (Moscow), 2003, vol. 50, pp. 485–495 (Russ. J. Plant Physiol., Engl. Transl., pp. 431–440).
Mi, H., Deng, Y., Tanaka, Y., Hibino, T., and Takabe, T., Photo-Induction of an NADPH Dehydrogenase Which Functions as a Mediator of Electron Transport to the Intersystem Chain in the Cyanobacterium Synechocystis PCC6803, Photosynth. Res., 2001, vol. 70, pp. 167–173.
Fork, D.C. and Herbert, S., The Application of Photoacoustic Techniques to Studies of Photosynthesis, Photochem. Photobiol., 1993, vol. 57, pp. 207–220.
Leidreiter, K., Kruse, A., Riens, B., Winter, H., Lohaus, G., Robinson, D., Heineke, D., and Heldt, H.W., Subcellular Compartmentation of Metabolites in Plant Cells, Photosynthesis: From Light to Biosphere, Mathis, P., Ed., Dordrecht: Kluwer, 1995, pp. 483–486.
Author information
Authors and Affiliations
Additional information
Original Russian Text © E.A. Egorova, N.G. Bukhov, A.G. Shugaev, D.A. Los, 2006, published in Fiziologiya Rastenii, 2006, Vol. 53, No. 3, pp. 336–342.
Rights and permissions
About this article
Cite this article
Egorova, E.A., Bukhov, N.G., Shugaev, A.G. et al. Effect of exogenous glucose on electron flow to photosystem I and respiration in cyanobacterial cells. Russ J Plant Physiol 53, 298–304 (2006). https://doi.org/10.1134/S1021443706030022
Received:
Issue Date:
DOI: https://doi.org/10.1134/S1021443706030022