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The role of adenosine A2A receptors of the preoptic area in somnogenic activity of 70 kDa protein in pigeons

  • Comparative and Ontogenic Physiology
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Abstract

The effects of adenosine A2A receptor antagonist microinjections into the hypothalamic ventrolateral preoptic area (VLPA) on the natural sleep-wake cycle and somnogenic activity of a 70 kDa heat shock protein (Hsp70) are analyzed electrophysiologically in birds, specifically, pigeons (Columba livia) for the first time. We report that: (1) at the beginning of the rest period, microinjections of 8-(3-Chlorostyryl)caffeine (ChC), an adenosine A2A receptor antagonist, into VLPA increase wake duration and inhibited sleep in a dose-dependent manner; (2) Hsp70 injections into VLPA evoke the somnogenic effect manifested as an increase in total slow-wave sleep (SWS) duration and enhancement of the mechanisms responsible for SWS triggering and maintenance; (3) ChC blockade of adenosine A2A receptors inhibits Hsp70-induced SWS. These data suggest that adenosine A2A receptors located in VLPA are involved in the modulation of the natural sleep-wake cycle and promotion of the Hsp70 somnogenic effect in pigeons. It is hypothesized that the somnogenic effect of Hsp70 is mediated by the modulating influence of this chaperone on the functioning of adenosine receptor proteins.

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Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    I. V. Ekimova & Yu. F. Pastukhov

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  1. I. V. Ekimova
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  2. Yu. F. Pastukhov
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Correspondence to I. V. Ekimova.

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Original Russian Text © I.V. Ekimova, Yu.F. Pastukhov, 2014, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2014, Vol. 50, No. 6, pp. 428–434.

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Ekimova, I.V., Pastukhov, Y.F. The role of adenosine A2A receptors of the preoptic area in somnogenic activity of 70 kDa protein in pigeons. J Evol Biochem Phys 50, 492–499 (2014). https://doi.org/10.1134/S0022093014060039

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  • DOI: https://doi.org/10.1134/S0022093014060039

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