Skip to main content
SpringerLink
Log in

Luminescence analysis of the structure of human alpha-1-microglobulin

  • Molecular Biophysics
  • Published:
Biophysics Aims and scope Submit manuscript

Abstract

Absorption, fluorescence, and fluorescence excitation spectra in UV and visible regions are studied for alpha-1-microglobulin preparations isolated from human urine by gel chromatography and immunoaffinity chromatography with charcoal adsorption. The possible nature of low-molecular-weight compounds that impart yellow-brown color to alpha-1-microglobulin preparations and their role in the stabilization of the structure of protein globule is discussed. The effect of urea (1–10 M) and guanidine hydrochloride (0.25–6 M) on the conformational state and fast internal dynamics of alpha-1-microglobulin is studied by tryptophan fluorescence. The unfolding of the protein under the action of denaturants is attended with pronounced activation of its nanosecond internal dynamics. Alpha-1-microglobulin can regain the initial conformation and internal dynamics typical of native protein after denaturation unfolding of the globule with 10 M urea or 6 M guanidine hydrochloride. Alpha-1-microglobulin isolated by gel chromatography can exist in a partially folded thermodynamically stable state in 4–6 M urea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

A1M:

alpha-1-microglobulin

GdCl:

guanidine hydrochloride

GF:

gel filtration

IC:

immunoadsorption/charcoal

RTTP:

room-temperature tryptophan phosphorescence

References

  1. D. R. Flower, Biochem. J. 318, 1 (1996).

    Google Scholar 

  2. M. Allhorn, A. Klapyta, and B. Åkerström, Free Radic. Biol. Med. 38, 557 (2005).

    Article  Google Scholar 

  3. M. Newcomer and D. Ong, Biochim. Biophys. Acta. 1482, 57 (2000).

    Google Scholar 

  4. M. Allhorn, T. Berggard, J. Nordberg, et al., Blood. 99, 1894 (2002).

    Article  Google Scholar 

  5. B. Åkerström, L. Logdberg, T. Berggard, et al., Biochim. Biophys. Acta. 1482, 172 (2000).

    Google Scholar 

  6. T. Berggard, N. Thelin, C. Falkenberg, et al., Eur. J. Biochem. 245(3), 676 (1997).

    Article  Google Scholar 

  7. Grubb, Clin. Nephrol. 38, S20 (1992).

    Google Scholar 

  8. J. Penders and J. R. Delanghe, Clinica Chimica Acta. 346, 107 (2004).

    Article  Google Scholar 

  9. Sala, M. Campagnoli, E. Perani, et al., J. Biol. Chem. 279, 51033 (2004).

    Article  Google Scholar 

  10. J. Larsson, M. Allhorn, and B. Åkerström, Archiv. Biochem. Biophys. 432, 196 (2004).

    Article  Google Scholar 

  11. M. Calero, J. Escribano, A. Grubb, and E. Mendez, J. Biol. Chem. 269, 384 (1994).

    Google Scholar 

  12. T. Berggard, A. Cohen, P. Perrson, et al., Protein Science. 8, 2611 (1999).

    Article  Google Scholar 

  13. L. Wester, M. U. Johansson, and B. Åkerström, Protein Expr. Purif. 11, 95 (1997).

    Article  Google Scholar 

  14. T. S. Serchenya, A. P. Drozhdenyuk, A. G. Pryadko et al., Vestsi Nats. Akad. Nav. Belarusi, ser. Khim. Nav. 2, 68 (2006).

    Google Scholar 

  15. B. Åkerström, T. Bratt, and J. J. Enghild, FEBS Lett. 362, 50 (1995).

    Article  Google Scholar 

  16. U. K. Laemmli, Nature 227, 680 (1970).

    Article  ADS  Google Scholar 

  17. V. M. Mazhul’ and D. G. Shcherbin, Biofizika 43, 456 (1998).

    Google Scholar 

  18. V. M. Mazhul’, Yu. S. Ermolaev, V. A. Bobrov et al., Vestsi AN BSSR, Ser. Biyal. Nav. No. 6, 52 (1976).

  19. S. V. Konev, Electron-excited States of Biopolymers (Nauka i Tekhnika, Minsk, 1965) [in Russian].

    Google Scholar 

  20. E. A. Chernitskii, Luminescence and Structural Lability of Proteins in Solution and Cell (Nauka i Tekhnika, Minsk, 1972) [in Russian].

    Google Scholar 

  21. A. P. Demchenko, Luminescence and Protein Structure Dynamics (Naukova Dumka, Kiev, 1988) [in Russian].

    Google Scholar 

  22. V. M. Mazhul’, S. V. Konev, Yu. S. Ermolaev, et al., Biofizika 28, 980 (1983).

    Google Scholar 

  23. J. M. Vanderkooi, D. B. Calhoun, and S. W. Englander, Science 236, 568 (1987).

    Article  ADS  Google Scholar 

  24. G. B. Strambini, J. Mol. Liq. 42, 155 (1998).

    Article  Google Scholar 

  25. J. V. Mersol, D. G. Steel, and A. Gafni, Biophys. Chem. 48, 281 (1993).

    Article  Google Scholar 

  26. M. Gonnelli, G. B. Strambini, Biochemistry 34(42), 13847 (1995).

    Article  Google Scholar 

  27. V. M. Mazhul’, E. M. Zaitseva and D. G. Shcherbin, Biofizika 45, 965 (2000).

    Google Scholar 

  28. V. M. Mazhul, E. M. Zaitseva, M. M. Shavlovsky, et al., Biochemistry 42, 13551 (2003).

    Article  Google Scholar 

  29. V. M. Mazhul’, E. A. Chernitskii, and S. V. Konev, Biofizika 15, 5 (1970).

    Google Scholar 

  30. K. K. Turoverov and I. M. Kuznetsova, Mol. Biol. 17, 468 (1983).

    Google Scholar 

  31. I. M. Kuznetsova, Doctoral Dissertation in Biology (Institute of Cytology, RAS, St. Petersburg, 2006).

    Google Scholar 

  32. V. M. Mazhul’ and S. Zh. Kananovich, Biofizika 49, 413 (2004).

    Google Scholar 

  33. E. A. Chernitskii, V. M. Mazhul’, and S. V. Konev, Biofizika 13, 581 (1968).

    Google Scholar 

  34. D. A. Dolgikh, R. I. Gilmanshin, E. V. Brazhnikov, et al., FEBS Lett. 136, 311 (1981).

    Article  Google Scholar 

  35. M. Ohgushi and A. Wada, FEBS Lett. 164, 21 (1983).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, 220072, Belarus

    V. M. Mazhul & S. Zh. Kananovich

  2. Institute of Bioorganic Chemistry National Academy of Sciences of Belarus, Minsk, 220141, Belarus

    T. S. Serchenya & O. V. Sviridov

Authors
  1. V. M. Mazhul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Zh. Kananovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. S. Serchenya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. O. V. Sviridov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. M. Mazhul.

Additional information

Original Russian Text © V.M. Mazhul, S.Zh. Kananovich T.S. Serchenya O.V. Sviridov, 2007, published in Biofizika, 2007, Vol. 52, No. 3, pp. 425–435.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mazhul, V.M., Kananovich, S.Z., Serchenya, T.S. et al. Luminescence analysis of the structure of human alpha-1-microglobulin. BIOPHYSICS 52, 268–276 (2007). https://doi.org/10.1134/S0006350907030025

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006350907030025

Key words

Search

Navigation