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Investigation of stability of photosynthetic reaction center and quantum dot hybrid films

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Abstract

The efficiency of interaction (efficiency of energy transfer) between various quantum dots (QDs) and photosynthetic reaction centers (RCs) from the purple bacterium Rhodobacter sphaeroides and conditions of long-term stability of functioning of such hybrid complexes in film preparations were investigated. It was found that dry films containing RCs and QDs and maintained at atmospheric humidity are capable to keep their functional activity for at least some months as judging by results of measurement of their spectral characteristics, efficiency of energy transfer from QDs to RCs, and RC electron-transport activity. Addition of trehalose to the films giving them still greater stability is especially expressed for films maintained at low humidity. These stable hybrid film structures are promising for further biotechnological studies for developing new phototransformation devices.

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Abbreviations

BChl:

bacteriochlorophyll

BPheo:

bacteriopheophytin

LDAO:

lauryldimethylamine oxide

P:

photoactive BChl dimer

P+ :

oxidized BChl dimer

QA :

primary quinone acceptor

QB :

secondary quinone

QD:

quantum dots

Qx :

absorption band of P with maximum about 600 nm

RC:

reaction center

τ:

characteristic fluorescence time

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Authors and Affiliations

  1. Lomonosov Moscow State University, Biological Faculty, 119991, Moscow, Russia

    E. P. Lukashev, P. P. Knox, I. P. Oleinikov, N. Kh. Seifullina & N. P. Grishanova

Authors
  1. E. P. Lukashev
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  2. P. P. Knox
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  3. I. P. Oleinikov
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  4. N. Kh. Seifullina
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  5. N. P. Grishanova
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Correspondence to P. P. Knox.

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Original Russian Text © E. P. Lukashev, P. P. Knox, I. P. Oleinikov, N. Kh. Seifullina, N. P. Grishanova, 2016, published in Biokhimiya, 2016, Vol. 81, No. 1, pp. 135-142.

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Lukashev, E.P., Knox, P.P., Oleinikov, I.P. et al. Investigation of stability of photosynthetic reaction center and quantum dot hybrid films. Biochemistry Moscow 81, 58–63 (2016). https://doi.org/10.1134/S0006297916010065

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  • DOI: https://doi.org/10.1134/S0006297916010065

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