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Influence of fucoidans and their derivatives on antitumor and phagocytic activity of human blood leucocytes

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Abstract

The immunotropic activity of structurally different fucoidans and their derivatives towards isolated immune blood cells, effectors of innate immune system, was studied. The most potent effect was observed for high molecular weight fucoidan CF from the alga Chordaria flagelliformis, whose backbone is built of (1→3)-linked units of α-L-fucopyranose, and branches included residues of α-D-glucuronic acid and α-L-fucofuranose. This compound at the concentration of 0.05 mg/ml potentiated phagocytosis of Saccharomyces cerevisiae and Lactobacillus acidophilus by neutrophils, increasing relative quantity of phagocytes as well as their effectiveness. Along with this, 14% increase in the concentration of membrane-bound integrin CD11c molecules was observed. The systemic effect of CF at the dose of 0.01 mg/mouse i.p. led to potentiation of cytotoxic activity of spleen mononuclear leucocytes towards melanoma cells of line B16 by 1.9-fold and towards chronic myelogenous leukemia cells of line K-562 by 1.7-fold. These results indicate that fucoidan CF can stimulate anti-infective and antitumor activity of effectors of the innate immune system via CD11c integrins.

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Abbreviations

CF:

high molecular weight fucoidan from alga Chordaria flagelliformis

DMSO:

dimethyl sulfoxide

EC:

effector cells

IC:

index of cytotoxicity

MFI:

mean fluorescence intensity

ML:

mononuclear leukocytes

NBT:

nitroblue tetrazolium

NK:

natural killer cells

OS:

synthetic fully sulfated octasaccharide

PI:

phagocytic index

PN:

phagocytic number

PPdX:

dexylosylated low molecular weight fucoidan from alga Punctaria plantaginea

SL:

high molecular weight fucoidan from alga Saccharina latissima

TC:

target cells

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Authors and Affiliations

  1. Blokhin Russian Cancer Research Center, 115478, Moscow, Russia

    N. Yu. Anisimova, F. V. Donenko & M. V. Kiselevskiy

  2. Zelinsky Institute of Organic Chemistry, 119991, Moscow, Russia

    N. E. Ustyuzhanina, M. I. Bilan, A. I. Usov & N. E. Nifantiev

  3. Orekhovich Institute of Biomedical Chemistry, 119121, Moscow, Russia

    N. A. Ushakova

Authors
  1. N. Yu. Anisimova
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  2. N. E. Ustyuzhanina
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  3. F. V. Donenko
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  4. M. I. Bilan
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  5. N. A. Ushakova
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  6. A. I. Usov
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  7. N. E. Nifantiev
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  8. M. V. Kiselevskiy
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Correspondence to N. Yu. Anisimova or N. E. Ustyuzhanina.

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Original Russian Text © N. Yu. Anisimova, N. E. Ustyuzhanina, F. V. Donenko, M. I. Bilan, N. A. Ushakova, A. I. Usov, N. E. Nifantiev, M. V. Kiselevskiy, 2015, published in Biokhimiya, 2015, Vol. 80, No. 7, pp. 1099-1108.

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Anisimova, N.Y., Ustyuzhanina, N.E., Donenko, F.V. et al. Influence of fucoidans and their derivatives on antitumor and phagocytic activity of human blood leucocytes. Biochemistry Moscow 80, 925–933 (2015). https://doi.org/10.1134/S0006297915070111

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  • DOI: https://doi.org/10.1134/S0006297915070111

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