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Photobiosensors containing luminescent bacteria

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Abstract

The scientific basis for producing luminescent biosensors containing free and immobilized luminescent bacteria is discussed. Modern technologies for engineering target objects, procedures used to immobilize bacteria in different carriers, as well as procedures for integral and specific biodetection of toxins are presented. Data regarding generation and application of biomonitoring for ecotoxicants derived from natural and genetically engineered photobacterial strains are analyzed. Special attention is given to immobilization of photobacteria in polyvinyl alcohol-containing cryogel. The main physicochemical, biochemical, and technological parameters for stabilizing luminescence in immobilized bacteria are described. Results of the application of immobilized photobacterial preparations both during discrete and continuous biomonitoring for different classes of ecotoxicants are presented.

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Authors and Affiliations

  1. Faculty of Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    A. D. Ismailov & L. E. Aleskerova

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  1. A. D. Ismailov
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  2. L. E. Aleskerova
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Correspondence to A. D. Ismailov.

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Original Russian Text © A. D. Ismailov, L. E. Aleskerova, 2015, published in Biokhimiya, 2015, Vol. 80, No. 6, pp. 867–881.

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Ismailov, A.D., Aleskerova, L.E. Photobiosensors containing luminescent bacteria. Biochemistry Moscow 80, 733–744 (2015). https://doi.org/10.1134/S0006297915060085

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  • DOI: https://doi.org/10.1134/S0006297915060085

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