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Normal level of sepsis-associated phenylcarboxylic acids in human serum

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Abstract

Previous studies showed that large amounts of phenylcarboxylic acids (PhCAs) are accumulated in a septic patient’s blood due to increased endogenous and microbial phenylalanine and tyrosine biotransformation. Frequently, biochemical aromatic amino acid transformation into PhCAs is considered functionally insignificant for people without monogenetic hereditary diseases. The blood of healthy people contains the same PhCAs that are typical for septic patients as shown in this paper. The overall serum PhCAs level was 6 μM on average as measured by gas chromatography with flame ionization detection. This level is a stable biochemical parameter indicating the normal metabolism of aromatic amino acids. The concentrations of PhCAs in the metabolic profile of healthy people are distributed as follows: phenylacetic ≈ p-hydroxyphenyllactic > p-hydroxyphenylacetic > phenyllactic ≈ phenylpropionic > benzoic. We conclude that maintaining of stable PhCAs level in the serum is provided as the result of integration of human endogenous metabolic pathways and microbiota.

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Abbreviations

BA:

benzoic acid

3,4-DHBA:

3,4-dihydroxybenzoic acid

GC-FID:

gas chromatography with flame ionization detection

GC-MS:

gas chromatography coupled with mass spectrometry

IR:

interquartile range

PAA:

phenylacetic acid

PhCAs:

phenylcarboxylic acids

p-HPAA:

p-hydroxyphenylacetic acid

p-HPPA:

p-hydroxyphenylpropionic acid

p-HPLA:

p-hydroxyphenyllactic acid

PLA:

phenyllactic acid

PPA:

phenylpropionic acid

PR:

percentile range

TMS:

trimethylsilyl

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Authors and Affiliations

  1. Negovsky Research Institute of General Reanimatology, ul. Petrovka 25/2, 107031, Moscow, Russia

    N. V. Beloborodova, V. V. Moroz, A. A. Osipov, A. Yu. Bedova, A. Yu. Olenin & M. L. Getsina

  2. Dmitry Rogachev Federal Scientific Clinical Center of Pediatric Hematology, Oncology, and Immunology, Ministry of Health of the Russian Federation, ul. Samora Mashel 1, 117198, Moscow, Russia

    A. A. Osipov & O. V. Karpova

  3. All-Russian Research Institute of Metrological Service, ul. Ozernaya 46, 119361, Moscow, Russia

    E. G. Olenina

Authors
  1. N. V. Beloborodova
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  2. V. V. Moroz
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  3. A. A. Osipov
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  4. A. Yu. Bedova
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  5. A. Yu. Olenin
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  6. M. L. Getsina
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  7. O. V. Karpova
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  8. E. G. Olenina
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Corresponding author

Correspondence to N. V. Beloborodova.

Additional information

Published in Russian in Biokhimiya, 2015, Vol. 80, No. 3, pp. 449–455.

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Beloborodova, N.V., Moroz, V.V., Osipov, A.A. et al. Normal level of sepsis-associated phenylcarboxylic acids in human serum. Biochemistry Moscow 80, 374–378 (2015). https://doi.org/10.1134/S0006297915030128

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  • DOI: https://doi.org/10.1134/S0006297915030128

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