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RETRACTED ARTICLE: Human leptin triggers proliferation of A549 cells via blocking endoplasmic reticulum stress-related apoptosis

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This article was retracted on 13 December 2014

Abstract

Lung cancer is a disease characterized by uncontrolled cell growth in tissues of the lung. Leptin is a pleiotropic hormone with antiapoptotic and proliferative roles involved in several systems. However, there is no known antiapoptotic mechanism of leptin in non-small cell lung cancer (NSCLC). So, we investigated the antiapoptotic mechanism of leptin in NSCLC. Proliferation, apoptosis, and the specific mechanism of leptin-transfected cells were analyzed in this study. Leptin, p-Perk, IRE1, cleaved ATF6, spliced XBP1, eIF2-α, TRAF2, CHOP, and caspase 12 proteins were detected by Western blot, and endoplasmic reticulum (ER) stress-related mRNA was detected by semi-quantitative reverse transcription PCR (RT-PCR). Leptin in A549 and transfected cells inhibited cisplatin-activated ER stress-associated mRNA transcription and activation of proteins. ER stress unfolded protein response (UPR) proteins, PERK and ATF6, were involved in leptin-triggered apoptosis. XBP1 and TRAF2 were increased significantly when treated with cisplatin in A549-siLPT and non-transfected cells. CHOP expression was blocked in A549 and transfected cells (LPT-PeP and LPT-EX cells). In conclusion, leptin can promote the proliferation of A549 cells through blocking ER stress-mediated apoptosis. This blocking is mediated by the p-Perk and ATF6 pathway through blocking activation of CHOP.

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Authors and Affiliations

  1. Guangzhou Institute of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, 510120, China

    Wei Wang

  2. Neurosurgery, Affiliated Hospital of Inner Mongolia Medical University, Huhehaote, 010051, China

    Haicheng Yan, Changwu Dou & Youle Su

Authors
  1. Wei Wang
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  2. Haicheng Yan
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  3. Changwu Dou
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  4. Youle Su
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Corresponding author

Correspondence to Changwu Dou.

Additional information

Published in Russian in Biokhimiya, 2013, Vol. 78, No. 12, pp. 1684–1694.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM13-135, August 25, 2013.

These authors equally contributed to this work.

The article published in Vol. 78, No. 12, pp. 1333_1341, DOI: 10.1134/S0006297913120031, has been retracted by the authors request. The authors have recently obtained new results conflicting with those presented in the published article. Careful analysis showed that some published results were obtained using inappropriate methods and wrong statistical analysis. The authors apologize and ask readers to ignore the results published in this article.

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Wang, W., Yan, H., Dou, C. et al. RETRACTED ARTICLE: Human leptin triggers proliferation of A549 cells via blocking endoplasmic reticulum stress-related apoptosis. Biochemistry Moscow 78, 1333–1341 (2013). https://doi.org/10.1134/S0006297913120031

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  • DOI: https://doi.org/10.1134/S0006297913120031

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