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Oxidative phosphorylation and respiratory control phenomenon in Paracoccus denitrificans plasma membrane

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Abstract

Changes in respiratory activity, transmembrane electric potential, and ATP synthesis as induced by additions of limited amounts of ADP and Pi to tightly coupled inverted (inside-out) Paracoccus denitrificans plasma membrane vesicles were traced. The pattern of the changes was qualitatively the same as those observed for coupled mitochondria during the classical State 4-State 3-State 4 transition. Bacterial vesicles devoid of energy-dependent permeability barriers for the substrates of oxidation and phosphorylation were used as a simple experimental model to investigate two possible mechanisms of respiratory control: (i) in State 4 phosphoryl transfer potential (ATP/ADP × Pi) is equilibrated with proton-motive force by reversibly operating F1·Fo-ATPase (thermodynamic control); (ii) in State 4 apparent “equilibrium” is reached by unidirectional operation of proton motive force-activated F1·Fo-ATP synthase. The data support the kinetic mechanism of the respiratory control phenomenon.

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Abbreviations

\(\Delta \tilde \mu _{{\rm H}^ + }\) :

transmembrane electrochemical gradient of protons

F1 and Fo :

hydrophilic and hydrophobic parts of ATP synthase, respectively

FCCP:

carbonyl cyanide p-trifluoromethoxyphenylhydrazone

p :

proton motive force (p = ΔΨ − ΔpH) where ΔΨ is a transmembrane electric potential and ΔpH is pH difference between inner (matrix) and outer space of mitochondria

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    T. V. Zharova & A. D. Vinogradov

Authors
  1. T. V. Zharova
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  2. A. D. Vinogradov
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Correspondence to A. D. Vinogradov.

Additional information

Published in Russian in Biokhimiya, 2012, Vol. 77, No. 9, pp. 1207–1215.

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Zharova, T.V., Vinogradov, A.D. Oxidative phosphorylation and respiratory control phenomenon in Paracoccus denitrificans plasma membrane. Biochemistry Moscow 77, 1000–1007 (2012). https://doi.org/10.1134/S0006297912090064

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  • DOI: https://doi.org/10.1134/S0006297912090064

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