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Carbohydrate specificity of chicken and human tandem-repeat-type galectins-8 in composition of cells

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Abstract

The network of adhesion/growth-regulatory galectins in chicken (chicken galectin, CG) has only one tandemrepeat-type protein, CG8. Using a cell-based assay and probing galectin reactivity with a panel of fluorescent neoglycoconjugates (glycoprobes), its glycan-binding profile was determined. For internal validation, human galectin-8 (HG8) was tested. In comparison to HG8, CG8 showed a rather similar specificity: both galectins displayed high affinity to blood group ABH antigens as well as to 3′-sialylated and 3′-sulfated lactosamine chains. The most remarkable difference was found to be an ability of HG8 (but not CG8) to bind the disaccharide Galβ1-3GlcNAc (Lec) as well as branched and linear oligolactosamines. The glycan-binding profile was shown to be influenced by glycocalix of the cell, where the galectin is anchored. Particularly, glycosidase treatment of galectin-loaded cells led to the change of the profile. Thus, we suppose the involvement of cis-glycans in the interaction of cell-anchored galectins with external glycoconjugates.

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Abbreviations

BSA:

bovine serum albumin

CG8:

chicken galectin-8

CRD:

carbohydrate recognition domain

FITC:

fluorescein isothiocyanate

Glyc-PAA:

conjugate of glycan with polyacrylamide

HG8:

human galectin-8

PBA:

phosphatebuffered saline (pH 7.2) containing 0.2% BSA

PBS:

phosphate-buffered saline (pH 7.2)

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    O. A. Vokhmyanina, E. M. Rapoport, I. M. Ryzhov, E. Yu. Korchagina, G. V. Pazynina, V. V. Severov & N. V. Bovin

  2. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinarstr. 13, D-80539, Munich, Germany

    H. Kaltner, S. André & H. -J. Gabius

Authors
  1. O. A. Vokhmyanina
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  2. E. M. Rapoport
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  3. I. M. Ryzhov
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  4. E. Yu. Korchagina
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  5. G. V. Pazynina
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  6. V. V. Severov
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  7. H. Kaltner
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  8. S. André
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  9. H. -J. Gabius
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  10. N. V. Bovin
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Corresponding author

Correspondence to N. V. Bovin.

Additional information

Published in Russian in Biokhimiya, 2011, Vol. 76, No. 10, pp. 1452–1460.

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Vokhmyanina, O.A., Rapoport, E.M., Ryzhov, I.M. et al. Carbohydrate specificity of chicken and human tandem-repeat-type galectins-8 in composition of cells. Biochemistry Moscow 76, 1185–1192 (2011). https://doi.org/10.1134/S0006297911100130

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  • DOI: https://doi.org/10.1134/S0006297911100130

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