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Microcystin-induced 8-hydroxydeoxyguanosine in DNA and its reduction by melatonin, vitamin C, and vitamin E in mice

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Abstract

Microcystin LR (MC-LR), a liver-specific toxin synthesized by Microcystis aeruginosa, was investigated. MC-LR initiated reactive oxygen species formation followed by damaging DNA and some other cellular components. We investigated the ability of MC-LR to induce oxidative DNA damage by examining the formation of 8-hydroxydeoxyguanosine (8-OH-dG) using HPLC with electrochemical detection. Melatonin, vitamin C (ascorbate), and vitamin E (as Trolox), all of which are free radical scavengers, markedly inhibited the formation of 8-OH-dG in a concentration-dependent manner. The concentration that reduced DNA damage by 50% (IC50) was 0.55, 31.4, and 36.8 μM for melatonin, ascorbate, and Trolox, respectively. The results show that melatonin is 60-and 70-fold more effective than vitamin C or vitamin E, respectively, in reducing oxidative DNA damage. These findings are consistent with the conclusion that melatonin’s highly protective effect against microcystin toxicity relates, at least in part, to its direct hydroxyl radical scavenging ability.

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Abbreviations

ALT:

alanine transaminase

8-OH-dG:

8-hydroxydeoxyguanosine

References

  1. Pinho, G. L., Rosa, C., Yunes, J. S., Luquet, C. M., Bianchini, A., and Monserrat, J. M. (2003) Comp. Biochem. Physiol. Part C, 135, 459–468.

    CAS  Google Scholar 

  2. Ding, W. X., Shen, H. M., and Ong, C. N. (2000) Environ. Health Perspect., 108, 605–609.

    PubMed  CAS  Google Scholar 

  3. Carmichael, W. W. (1994) Sci. Am., 270, 64–72.

    Google Scholar 

  4. Gehringer, M. M., Shephard, E. G., Downing, T. G., and Neilan, B. A. (2004) Int. J. Biochem. Cell Biol. 36, 931–941.

    Article  PubMed  CAS  Google Scholar 

  5. Ding, W. X., Shen, H. M., and Ong, C. N. (2001) J. Toxicol. Environ. Health. Part A, 64, 507–519.

    Article  PubMed  CAS  Google Scholar 

  6. Aust, A. E., and Eveligh, J. (1999) Exp. Biol. Med., 43, 246–252.

    Article  Google Scholar 

  7. Qi, W., Reiter, R. J., Tan, D., Garcia, J. J., Manchester, L. C., Karbownik, M., and Calvo, J. R. (2000) Environ. Health Perspect., 108, 398–402.

    Google Scholar 

  8. Cheng, K. C., Cahill, D. S., Kasai, H., Nishimura, S., and Loeb, L. A. (1992) J. Biol. Chem., 267, 166–172.

    PubMed  CAS  Google Scholar 

  9. Asami, S., Manabe, H., Miyake, J., Tsurudome, Y., Hirano, T., Yamaguchi, R., Itoh, H., and Kasai, H. (1997) Carcinogenesis, 18, 1763–1766.

    Article  PubMed  CAS  Google Scholar 

  10. Lloyd, D. R., Carmichael, P. L., and Phillips, D. H. (1998) Chem. Res. Toxicol., 11, 420–427.

    Article  PubMed  CAS  Google Scholar 

  11. Ding, W. X., and Ong, C. N. (2003) FEMS Microbiol. Lett., 220, 1–7.

    Article  PubMed  CAS  Google Scholar 

  12. Kuo, H. W., Cheng, S. F., Wu, K. Y., and Wu, F. Y. (2003) Occup. Environ. Med., 60, 590–594.

    Article  PubMed  CAS  Google Scholar 

  13. Reiter, R. J., Melchorri, D., Chuang, J. I., and Oritz, G. G. (1995) J. Pineal Res., 18, 1–11.

    PubMed  CAS  Google Scholar 

  14. Karbownik, M., and Reiter, R. J. (2000) Exp. Biol. Med., 47, 9–22.

    Article  Google Scholar 

  15. Ferry, G., Ubeaud, C., Mozo, J., Pean, C., Galizzi, P., Philippe, D., Renard, P., Yons, S., Lesieur, D., and Boutin, J. (2004) Eur. J. Biochem., 271, 418–428.

    Article  PubMed  CAS  Google Scholar 

  16. Fischer, C. P., Hiscock, N. J., Penkowa, M., Basu, S., Vessby, B., and Pedersen, B. (2004) J. Physiol., 558, 633–645.

    Article  PubMed  CAS  Google Scholar 

  17. Costa, E. J. X., Lopes, R. H., and Lamy, F. (1994) J. Pineal Res., 16, 123–126.

    Google Scholar 

  18. Shida, C. S., Castrucci, A. M., and Lamy, M. T. (1994) J. Pineal Res., 16, 198–201.

    PubMed  CAS  Google Scholar 

  19. Black, H. S. (1974) Res. Commun. Chem. Pathol. Pharmacol., 7, 783–786.

    PubMed  CAS  Google Scholar 

  20. Ames, B. N. (1989) Free Rad. Res. Commun., 7, 121–128.

    CAS  Google Scholar 

  21. Wu, T., Hashimoto, N., Wu, J., Carey, D., Li, R., Mickle, D., and Weisel, R. (1990) Biochem. Cell Biol., 68, 1189–1194.

    Article  PubMed  CAS  Google Scholar 

  22. Cerutti, C. A. (1985) Science, 227, 375–380.

    Article  PubMed  CAS  Google Scholar 

  23. Reiter, R. J., Guerrero, J. M., Garcia, J. J., and Acuna, D. (1998) Ann. N. Y. Acad. Sci., 854, 410–424.

    Article  PubMed  CAS  Google Scholar 

  24. Sies, H., and Stahl, W. (1995) Am. J. Clin. Nutr., 62, 1315–1321.

    Google Scholar 

  25. Floyd, R. A., West, M. S., Eneff, K. L., Hogsett, W. E., and Tingey, D. T. (1988) Arch. Biochem. Biophys., 262, 266–272.

    Article  PubMed  CAS  Google Scholar 

  26. Gehringer, M. M., Downs, K. S., Downing, T. G., Naude, R. J., and Shephard, E. G. (2003) Toxicon, 41, 451–458.

    Article  PubMed  CAS  Google Scholar 

  27. Gehringer, M. M., Govender, S., Shah, M., and Downing, T. G. (2003) Exp. Toxicol., 18, 142–148.

    Article  CAS  Google Scholar 

  28. Yoshida, T., Makita, Y., Nagata, S., Tsutsumi, T., Yoshida, F., Tamaru, S., and Ucno, Y. (1997) Nat. Toxins, 5, 91–95.

    Article  PubMed  CAS  Google Scholar 

  29. Tsou, T. C., Chen, C. L., Liu, T. Y., and Yang, J. L. (1996) Carcinogenesis, 17, 102–108.

    Google Scholar 

  30. Ding, W. X., Shen, H. M., Zhu, H. G., and Ong, C. N. (1998) Environ. Res., 78, 12–18.

    Article  PubMed  CAS  Google Scholar 

  31. Rao, P. V. L., Gupta, N., and Jayaraj, R. (2004) Indian J. Pharmacol., 36, 87–92.

    CAS  Google Scholar 

  32. Stewart, M. S., Cameron, G. S., and Pence, B. C. (1996) J. Invest. Dermatol., 106, 1086–1089.

    Article  PubMed  CAS  Google Scholar 

  33. Toyokuni, S., and Sagripanti, J.-L. (1996) Free Rad. Biol. Med., 20, 859–864.

    Article  PubMed  CAS  Google Scholar 

  34. Halliwell, B., and Aruoma, O. I. (1991) FEBS Lett., 281, 9–19.

    Article  PubMed  CAS  Google Scholar 

  35. Bissett, D. L., Chatterjee, R., and Hannon, D. P. (1990) Photodermatol. Photomed., 7, 56–92.

    CAS  Google Scholar 

  36. Shen, C. L., Song, W., and Pence, B. C. (2001) Epidemiol. Biomarkers Prev., 10, 385–390.

    CAS  Google Scholar 

  37. Fang, Y. Z., Yang, S., and Wu, G. (2002) Nutr., 18, 872–879.

    Article  CAS  Google Scholar 

  38. Mayo, J. C., Tan, D. X., Sainz, R. M., Burillo, S. L., and Reiter, R. J. (2003) Free Rad. Res., 37, 543–553.

    Article  CAS  Google Scholar 

  39. Tan, D., Chen, L. D., Manchester, L. C., and Retier, R. J. (1993) Endocrinol. J., 1, 57–60.

    Google Scholar 

  40. Pahkla, R., Zilmer, M., and Rago, L. (1998) J. Pineal Res., 24, 96–101.

    PubMed  CAS  Google Scholar 

  41. Stasica, P., Ulanskis, P., and Roseak, J. M. (1998) J. Pineal Res., 25, 65–66.

    PubMed  CAS  Google Scholar 

  42. Tan, D., Manchester, L. C., Reiter, R. J., Plummer, B. F., Hardies, L. J., and Weintraub, S. F. (1998) Biochem. Biophys. Res. Commun., 253, 614–620.

    Article  PubMed  CAS  Google Scholar 

  43. Limson, J., Nykong, T., and Daya, S. (1998) J. Pineal Res., 24, 15–21.

    PubMed  CAS  Google Scholar 

  44. Reiter, R. J. (1997) Adv. Pharmacol., 38, 103–117.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, Yarmouk University, Irbid, Jordan

    S. Al-Jassabi & A. M. Khalil

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  1. S. Al-Jassabi
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  2. A. M. Khalil
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Correspondence to S. Al-Jassabi.

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Published in Russian in Biokhimiya, 2006, Vol. 71, No. 10, pp. 1377–1382.

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Al-Jassabi, S., Khalil, A.M. Microcystin-induced 8-hydroxydeoxyguanosine in DNA and its reduction by melatonin, vitamin C, and vitamin E in mice. Biochemistry (Moscow) 71, 1115–1119 (2006). https://doi.org/10.1134/S0006297906100099

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  • DOI: https://doi.org/10.1134/S0006297906100099

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