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Characterization of biodegradable cell micro and macro carriers based on recombinant spidroin

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Abstract

Gels, microgels, and matrices were prepared based on a previously developed recombinant spidroin, 1F9, produced by Saccharomyces cerevisiae yeast strain; their physical, chemical, and biological properties were investigated. It was shown that microgels obtained from 2.5% hydrogel are sized in the range of 50–300 μm, with a predominance of particles of 50–150 μm in diameter. The microgel particles were stable in neutral, acidic, and alkaline media; the destruction of 50% (wt.) of the particles occurred in corrosive media (Fenton’s reagent) within three weeks. The microgel surface, which was studied by laser scanning confocal microscopy and scanning electron microscopy, has a complex relief in which there are both nano-(250–400 nm) and microstructures (3–7 μm). It was shown that particles of both microgel and matrices are capable of adhesion and support the proliferation of 3T3 murine fibroblasts in vitro.

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Abbreviations

EDTA:

ethylenediaminetetraacetate

EP:

electrophoresis

PAAG:

polyacrylamide gel

PBS:

phosphate buffer solution

SDS:

sodium dodecylsulfate

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Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    V. G. Bogush, L. I. Davydova, K. V. Sidoruk, D. G. Kozlov & V. G. Debabov

  2. Faculty of Biology, Moscow State University, Moscow, 119991, Russia

    M. M. Moisenovich, A. Yu. Arkhipova & M. P. Kirpichnikov

  3. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, 123182, Russia

    I. I. Agapov

Authors
  1. V. G. Bogush
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  2. L. I. Davydova
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  3. M. M. Moisenovich
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  4. K. V. Sidoruk
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  5. A. Yu. Arkhipova
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  6. D. G. Kozlov
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  7. I. I. Agapov
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  8. M. P. Kirpichnikov
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  9. V. G. Debabov
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Corresponding author

Correspondence to V. G. Bogush.

Additional information

Original Russian Text © V.G. Bogush, L.I. Davydova, M.M. Moisenovich, K.V. Sidoruk, A.Yu. Arkhipova, D.G. Kozlov, I.I. Agapov, M.P. Kirpichnikov, V.G. Debabov, 2014, published in Biotekhnologiya, 2014, No. 1, pp. 52–61.

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Bogush, V.G., Davydova, L.I., Moisenovich, M.M. et al. Characterization of biodegradable cell micro and macro carriers based on recombinant spidroin. Appl Biochem Microbiol 50, 780–788 (2014). https://doi.org/10.1134/S000368381408002X

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  • DOI: https://doi.org/10.1134/S000368381408002X

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