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Polystyrene microspheres as bioligand carriers in the determination of Δ-9-tetrahydrocannabinol in human urine

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Abstract

For the first time, a diagnostic test system for the determination of drugs, in particular, cannabinoids, in human urine was constructed on the basis of the latex agglutination reaction. For this purpose, polystyrene microspheres with a diameter of 0.6 μm and a narrow particle size distribution and carboxylic groups of stabilizer molecules on their surfaces were used. The studies revealed the optimal characteristics of particle suspension, which provide for a high sensitivity and specificity level of the test, in particular, the quantitative range of the antibody content for covalent binding with carboxylic groups on the surface of polystyrene microspheres. The operating titer of Δ-9-tetrahydrocannabinol (Δ-9-THC)-specific antibodies was 1/16-1/512 for a conjugated antigen taken at a concentration of 0.1 mg/mL. The sensitivity of the latex agglutination reaction when determining Δ-9-THC was 150 ng/mL.

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Abbreviations

ISA:

immunosorbent assay

ELISA:

enzymelinked immunosorbent assay

CA:

conjugated antigen

CS:

α,ω-bis[10-carboxydecyl]polydimethylsiloxane

MW:

molecular weight

PPS:

potassium persulphate

Δ-9-THC:

Δ-9-tetrahydrocannabinol

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Authors and Affiliations

  1. Lomonosov State Academy of Fine Chemical Technology, pr. Vernadskogo 86, Moscow, 119571, Russia

    N. M. Solodukhina, L. A. Zlydneva, E. N. Levshenko & I. A. Gritskova

  2. Institute of Physiologically Active Substances, Russian Academy of Sciences, Chernogolovka, Moscow oblast, 142432, Russia

    M. A. Myagkova

Authors
  1. N. M. Solodukhina
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  2. L. A. Zlydneva
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  3. E. N. Levshenko
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  4. M. A. Myagkova
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  5. I. A. Gritskova
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Correspondence to N. M. Solodukhina.

Additional information

Original Russian Text © N.M. Solodukhina, L.A. Zlydneva, E.N. Levshenko, M.A. Myagkova, I.A. Gritskova, 2012, published in Biotekhnologiya, 2012, No. 1, pp. 90–96.

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Solodukhina, N.M., Zlydneva, L.A., Levshenko, E.N. et al. Polystyrene microspheres as bioligand carriers in the determination of Δ-9-tetrahydrocannabinol in human urine. Appl Biochem Microbiol 48, 740–745 (2012). https://doi.org/10.1134/S0003683812090062

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  • DOI: https://doi.org/10.1134/S0003683812090062

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