Abstract
A novel method was developed to incorporate macrocyclic polyene antibiotics, nystatin and amphotericin B, into liposomes prepared from the mixture of phosphatidylcholine and cholesterol (7: 3) or phosphatidylcholine, cholesterol, and cardiolipin (7: 3: 1). Membranes of the liposomes were modified using the amphiphilic polymer N-vinylpyrrolidone with the molecular mass (MM) of the polymer fragment of 4000 and a single terminal n-octadecyl group. The content of the antibiotic incorporated within such nanosize liposomal carriers can reach 17–22%. The obtained modified liposomes, 150–200 nm in size, were more stable during prolonged storage and more resistant to various destructive factors, such as destabilizing agents (Triton X-100, ethanol) and ultrasound. The liposomal preparations showed higher antifungal activity than non-immobilized antifungal antibiotics.
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Original Russian Text © I.A. Yamskov, A.N. Kuskov, K.K. Babievsky, B.B. Berezin, M.A. Krayukhina, N.A. Samoylova, V.E. Tikhonov, M.I. Shtilman, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 44, No. 6, pp. 688–693.
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Yamskov, I.A., Kuskov, A.N., Babievsky, K.K. et al. Novel liposomal forms of antifungal antibiotics modified by amphiphilic polymers. Appl Biochem Microbiol 44, 624–628 (2008). https://doi.org/10.1134/S0003683808060112
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DOI: https://doi.org/10.1134/S0003683808060112