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Quantitative analysis of IAA in DR5::GUS transgenic arabidopsis plants

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Abstract

In the study of auxin transport, transgenic constructs, including DR5::GUS, are widely used for visualization of phytohormone localization. Previously we proposed a method for quantitative evaluation of the IAA content by histochemical staining for glucuronidase activity. In this work, this method was complemented by quantitative data on the content of IAA in plants obtained by gas chromatography-mass spectrometry (GC/MS), which allowed more accurate characterization of the lateral IAA gradient arising at the Arabidopsis thaliana (L.) Heynh (ecotype Columbia 0) root gravistimulation. Applied method of IAA analysis, combining GC/MS and histochemistry, can be used for quantitatification of the other plant hormone distribution in transgenic plants with the GUS reporter.

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Abbreviations

GC/MS:

gas chromatography with mass-selective detection

GUS:

reporter enzyme β-D-glucuronidase from Escherichia coli

RGB:

color model where the combination of red, green, and blue color (R, G, B) produces the final color of digital image pixel

TMS:

trimethylsilyl fragment (Si(CH3)3)

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Authors and Affiliations

  1. Department of Plant Physiology and Biochemistry, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia

    G. A. Pozhvanov, A. L. Shavarda & S. S. Medvedev

  2. Komarov Institute of Botany, Russian Academy of Sciences, St. Petersburg, Russia

    G. A. Pozhvanov & A. L. Shavarda

Authors
  1. G. A. Pozhvanov
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  2. A. L. Shavarda
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  3. S. S. Medvedev
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Correspondence to G. A. Pozhvanov.

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Original Russian Text © G.A. Pozhvanov, A.L. Shavarda, S.S. Medvedev, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 3, pp. 446–451.

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Pozhvanov, G.A., Shavarda, A.L. & Medvedev, S.S. Quantitative analysis of IAA in DR5::GUS transgenic arabidopsis plants. Russ J Plant Physiol 60, 431–436 (2013). https://doi.org/10.1134/S1021443713020179

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  • DOI: https://doi.org/10.1134/S1021443713020179

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