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Protein quantification

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Abstract

Protein quantification is an integral part of any investigation related to protein isolation, purification, characterization, and analysis. Although the methods considered in this lecture have multi-year history and applied widely in the laboratory practice, there are some crucial points, which must be taken into consideration while choosing the method permitting reliably and with a high specificity and reproducibility to quantify protein.

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Abbreviations

BCA:

bicinchoninic acid

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    N. S. Stepanchenko, G. V. Novikova & I. E. Moshkov

Authors
  1. N. S. Stepanchenko
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  2. G. V. Novikova
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  3. I. E. Moshkov
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Corresponding author

Correspondence to G. V. Novikova.

Additional information

Original Russian Text © N.S. Stepanchenko, G.V. Novikova, I.E. Moshkov, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 4, pp. 624–630.

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Stepanchenko, N.S., Novikova, G.V. & Moshkov, I.E. Protein quantification. Russ J Plant Physiol 58, 737–742 (2011). https://doi.org/10.1134/S1021443711040182

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  • DOI: https://doi.org/10.1134/S1021443711040182

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