Abstract
The epitope presentation system for the ectodomain of the M2 protein (M2e) of the influenza A virus was constructed on the basis of the cowpea mosaic virus (CPMV) for expression in the plant Vigna unguiculata. CPMV is widely used as a vector to produce immunogenic chimeric virus particles (CVPs) bearing epitopes of various infectious human and animal pathogens. To produce chimeric CPMV particles in plants, two binary vectors were constructed to bear a modified gene coding for the CPMV S-coat protein with insertions of M2e epitopes of human influenza and bird influenza viruses. Antigenic and immunogenic properties of CVPs were investigated in mice immunization experiments. CVPs were shown to induce anti-M2e IgG production and to partly protect mice against a challenge with low doses of the influenza virus. However, low infectivity and immunogenicity of chimeric CPMV particles indicate that the plant virus-based systems for M2e epitope presentation requires further optimization in order to use plants as a possible source of flu vaccines.
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References
Karpukhin G.I. 2001. Gripp (Influenza), 2nd. ed. St. Peterburg: Gippokrat.
Cox N.J., Subarao K. 1999. Influenza. Lancet. 354, 1277–1282.
Kiselev O.I., Marinich I.G., Sominina A.A. 2003. Gripp i drugie virusnye respiratornye infektsii: epidemiologiya, profilaktika, diagnostika i terapiya (Influenza and other viral respiratory infections: Epidemiology, prevention, diagnosis, and therapy). St. Petersburg: Ross. Akad. Med. Nauk.
Kiselev O.I., Tsymbalova L.M., Pokrovsky V.I. 2006. Construction of vaccines against influenza virus H5N1 in the world and in Russia: Current state. Zh. Mikrobiol. Epidemiol. Immunobiol. 5, 28–38.
Gerdil C. 2003. The annual production cycle for influenza vaccine. Vaccine. 21, 1776–1779.
Kiselev O.I., Blinov V.M., Kozel’skaya K.N., Il’enko V.I., Platonov V.G., Chupakhin O.N., Stukova M.A., Karginov V.A. 1993. Molecural action mechanism of adamantan-derived antiviral drugs. Vestn. Ross. Akad. Med. nauk. 3, 10–15.
Wanli L., Peng Z., Jian D., Ying-Hua C. 2005. Sequence comparison between the extracellular domain of M2 protein human and avian influenza A virus provides new information for bivalent influenza vaccine design. Microb Infect. 7, 171–177.
Neirynck S., Deroo T., Saelens X., Vanlandschoot P., Jou W.M., Fiers W. 1999. A universal influenza A vaccine based on the extracellular domain of the M2 protein. Nature Med. 5, 1157–1163.
De Filette M., Min Jou W., Birkett A., Lyons K., Schultz B., Tonkyro A., Resch S., Fiers W., 2005. Universal influenza A vaccine: Optimization of M2-based constructs. Virology. 337, 149–161.
Fiers W. 2006. A universal vaccine against influenza. Bull. Mem. Acad. R. Med. Belg. 161, 237–239.
Caizares M.C., Lomonossoff G.P., Nicholson L. 2005. Development of cowpea mosaic virus-based vectors for the production of vaccines in plants. Expert Rev. Vaccines. 4, 687–697.
Porta C., Spall V.E., Loveland J., Johnson J.E., Barker P.J., Lomonossoff G.P. 1994. Development of cowpea mosaic virus as a high-yielding system for the presentation of foreign peptides. Virology. 202, 949–955.
Lin T., Johnson J.E. 2003. Structures of picorna-like plant viruses: Implications and applications. Adv. Virus Res. 62, 167–239.
Lomonossoff G.P., Johnson J.E. 1996. Use of macromolecular assemblies as expression systems for peptides and synthetic vaccines. Curr. Opin. Struct. Biol. 6, 176–182.
Lin T., Porta C., Lomonossoff G., Johnson J.E. 1996. Structure-based design of peptide presentation on a viral surface: the crystal structure of a plant/animal virus chimera at 2.8 Å resolution. Fold Des. 1, 179–187.
Dalsgaard K., Uttenthal A., Jones T.D., Xu F., Merryweather A., Hamilton W.D., Langeveld J.P., Boshuizen R.S., Kamstrup S., Lomonossoff G.P., Porta C., Vela C., Casal J.I., Meloen R.H., Rodgers P.B. 1997. Plant-derived vaccine protects target animals against a viral disease. Nature Biotechnol. 15, 248–252.
Yasawardene S.G., Lomonossoff G.P., Ramasamy R. 2003. Expression and immunogenicity of malaria merozoite peptides displayed on the small coat protein of chimaeric cowpea mosaic virus. Indian J. Med. Res. 118, 115–124.
Liu L., Lomonossoff G.P. 2002. Agroinfection as a rapid method for propagating cowpea mosaic virus-based constructs. J. Virol. Methods. 105, 343–348.
Maniatis, T., Fritsch, E.F., Sambrook, J. 1982. Molecular Cloning: A Laboratory Manual. NY: Cold Spring Harbor Lab. Press.
Hoekema A., Hirsch P.R., Hooykaas P.J.J., Schilperoort R.A. 1983. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacteria tumefaciens Tiplasmid. Nature. 303, 179–180.
Crowther J.R. 2001. The Elisa Guidebook: Methods in Molecular Biology, vol. 149, Clifton, NJ: Humana Press.
McLain L., Porta C., Lomonossoff G.P., Durrani Z., Dimmock N.J. 1995. Human immunodeficiency virus type 1-neutralizing antibodies raised to a glycoprotein 41 peptide expressed on the surface of a plant virus. AIDS Res. Hum. Retroviruses. 11, 327–334.
Taylor K.M., Porta C., Lin T., Johnson J.E., Parker P.J., Lomonossoff G.P. 1999. Position-dependent processing of peptides presented on the surface of cowpea mosaic virus. Biol. Chem. 380, 387–392.
Nemchinov L.G., Natilla A. 2007. Transient expression of the ectodomain of matrix protein 2 (M2e) of avian influenza A virus in plants. Protein Exp. Purif. 56, 153–159.
Cleveland S.M., Jones T.D., Dimmock N.J. 2000. Properties of a neutralizing antibody that recognizes a conformational form of epitope ERDRD in the gp41 C-terminal tail of human immunodeficiency virus type 1. J. Gen. Virol. 81, 1251–1260.
Taylor K.M., Lin T., Porta C., Mosser A.G., Giesing H.A., Lomonossoff G.P., Johnson J.E. 2000. Influence of three-dimensional structure on the immunogenicity of a peptide expressed on the surface of a plant virus. J. Mol. Recognit. 13, 71–82.
Jegerlehner A., Schmitz N., Storni T., Bachmann M.F. 2004. Influenza A vaccine based on the extracellular domain of M2: Weak protection mediated via antibody-dependent NK cell activity. J. Immunol. 172, 5598–3605.
Denis J., Acosta-Ramirez E., Zhao Y., Hamelin M.E., Koukavica I., Baz M., Abed Y., Savard C., Pare C., Lopez Macias C., Boivin G., Leclerc D. 2008. Development of a universal influenza A vaccine based on the M2e peptide fused to the papaya mosaic virus (PapMV) vaccine platform. Vaccine. 26, 3395–3403.
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Original Russian Text © Yu.A. Meshcheryakova, M.A. Eldarov, A.I. Migunov, L.A. Stepanova, I.A. Repko, C.I. Kiselev, G.P. Lomonossoff, K.G. Skryabin, 2009, published in Molekulyarnaya Biologiya, 2009, Vol. 43, No. 4, pp. 751–760.
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Meshcheryakova, Y.A., Eldarov, M.A., Migunov, A.I. et al. Cowpea mosaic virus chimeric particles bearing the ectodomain of matrix protein 2 (M2E) of the influenza a virus: Production and characterization. Mol Biol 43, 685–694 (2009). https://doi.org/10.1134/S0026893309040219
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DOI: https://doi.org/10.1134/S0026893309040219