Biochemistry (Moscow)

, Volume 77, Issue 13, pp 1465–1477

Translation initiation in eukaryotes: Versatility of the scanning model

Authors

  • O. M. Alekhina
    • Institute of Protein ResearchRussian Academy of Sciences
    • Institute of Protein ResearchRussian Academy of Sciences
Review

DOI: 10.1134/S0006297912130056

Cite this article as:
Alekhina, O.M. & Vassilenko, K.S. Biochemistry Moscow (2012) 77: 1465. doi:10.1134/S0006297912130056

Abstract

It is generally accepted that the initiation of translation in eukaryotes involves the binding of the 40S ribosomal subunit to the capped 5′ end of an mRNA and subsequent scanning of 5′ UTR in search of an initiation codon. However, until recently this has remained a mere hypothesis. This review describes the novel experimental evidence in support of this classical model. Data on the participation of various factors in the eukaryotic initiation process are summarized. The sequence of initiation events is described in light of the latest experimental data. The existing physical models of scanning are presented. Special attention is paid to discussion of alternative models of eukaryotic initiation of translation. It is demonstrated that the canonical mechanism of initiation is more versatile than previously thought.

Key words

eukaryotesinitiation of translationscanning5′ UTRinitiation factor

Abbreviations

eIF

eukaryotic initiation factor

EMCV

encephalomyocarditis virus

IRES

internal ribosome entry site

PABP

poly(A) binding protein

UTR

untranslated region

Copyright information

© Pleiades Publishing, Ltd. 2012