Abstract
Conditions of cultivation and micropropagation of selected biotypes of five willow species (Salyx dasyclados Wimm., S. caspica Pall., S. triandra L., S. purpurea L., and S. viminalis L.) and two hybrids (×S. acuminata S. and ×S. palustris Host.) were optimized. Data on in vitro propagation of S. caspica, S. triandra, S. purpurea together with hybrids S. acuminata and S. palustris were obtained for the first time. It has been demonstrated that the outcome of cultivation and propagation of willows strongly depends on genotypic peculiarities of initial plants. The optimal terms of isolation and sterilization of single-node segments for obtaining 50–75% of aseptic viable developing cultures were estimated. The nutritive media were selected providing induction of stem development (to 67%), their rooting (to 91%), elongation (to 3–6 cm), and multiplication (propagation coefficient of 4). The designed method (adopted to different genotypes) can be applied for obtaining aseptic in vitro cultures serving as initial plant material for genetic transformation and mass propagation of plants with new agriculturally valuable characteristics which are of interest for construction of bioenergetic plantations and for needs of the paper industry.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BAP:
-
benzylaminapurine
- IAA:
-
3-indolylacetic acid
- NAA:
-
α-naphthylacetic acid
- MS:
-
murashige-fkoog medium
- 2-IP(2ip):
-
isopentenyladenine
- WPM:
-
woody plant medium
- GA3 :
-
gibberellic acid
References
Agrawal, D. and Gerbhardt, K., J. Plant Physiol., 1994, vol. 143, pp. 763–765.
Stoehr, M.U., Cai, M., and Zsuffa, Z., Can. J. For. Res., 1989, vol. 19, pp. 1634–1638.
Edson, J.L., Leege-Brusven, A.D., and Wenny, D.L., Tree Planters’ Notes, 1995, vol. 46, no. 2, pp. 58–63.
Bhojwani, S.S., New Zealand J. Botany, 1980, vol. 18, pp. 209–214.
Chalupa, V., Biologia Plantarum, 1983, vol. 25, no. 4, pp. 305–307.
Bergman, L., Arnold, S.V., and Eriksson, T., Plant Cell Tissue Organ Culture, 1985, vol. 4, no. 2, pp. 135–144.
Neuner, H. and Beiderbeck, R., Silvae Genetica, 1993, vol. 42, no. 6, pp. 308–310.
Amo-Marco, J.B. and Lledo, M.D., In vitro Cellular and Development Biology-Plant, 1996, vol. 32, no. 1, pp. 42–46.
Karkonen, A., Simola, L.K., and Koponen, T., Ann. Bot. Fennici, 1999, vol. 36, pp. 21–31.
Liesebach, M. and Naujoks, G., Plant Cell Tissue Organ Culture, 2004, vol. 79, no. 2, pp. 239–247.
Lyyra, S., Lima, A., and Merkle, S.A., Tree Physiol., 2006, vol. 26, pp. 969–975.
Park, S.Y., Plant Cell Tissue Organ Culture, 2008, vol. 93, no. 3, pp. 341–346.
Mashkina, O.S. and Tabatskaya, T.M., Rekomendatsii po sokhraneniyu i vosproizvodstvu metodami biotekhnologii tsennykh genotipov karel’skoi berezy, osiny, topolya belogo i sereyushchego (Guidelines to Conservation and Reproduction by Biotechnological Methods of Valuable Genotypes of Erman Birch, Aspen, and White and Gray Poplars), Voronezh: VGU, 2005.
Lloyd, G. and McCown, D., Comb. Proc. Intl. Plant. Prop. Soc., 1980, vol. 30, pp. 421–427.
Murashige, T.A. and Scoog, F.A., Phisiol. Plantarum, 1962, vol. 15, no. 3, pp. 473–497.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.S. Mashkina, T.M. Tabatskaya, A.I. Gorobets, K.A. Shestibratov, 2010, published in Biotekhnologiya, 2010, No. 1, pp. 65–73.
Rights and permissions
About this article
Cite this article
Mashkina, O.S., Tabatskaya, T.M., Gorobets, A.I. et al. Method of clonal micropropagation of different willow species and hybrids. Appl Biochem Microbiol 46, 769–775 (2010). https://doi.org/10.1134/S0003683810080065
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683810080065