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Three-dimensional structure of the enzyme dimanganese catalase from Thermus Thermophilus at 1 Å resolution

  • Structures of Macromolecular Compounds
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Abstract

The crystal structures of two forms of the enzyme dimanganese catalase from Thermus Thermophilus (native and inhibited by chloride) were studied by X-ray diffraction analysis at 1.05 and 0.98 Å resolution, respectively. The atomic models of the molecules were refined to the R factors 9.8 and 10%, respectively. The three-dimensional molecular structures are characterized in detail. The analysis of electron-density distributions in the active centers of the native and inhibited enzyme forms revealed that the most flexible side chains of the amino acid residues Lys162 and Glu36 exist in two interrelated conformations. This allowed us to obtain the structural data necessary for understanding the mechanism of enzymatic activity of the dimanganese catalase.

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Authors and Affiliations

  1. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow, 117333, Russia

    S. V. Antonyuk, V. R. Melik-Adamyan, A. N. Popov & V. V. Barynin

  2. European Molecular Biology Laboratory, EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, Hamburg, 22603, Germany

    A. N. Popov & V. S. Lamzin

  3. Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, P.O. Box 594, First Court, Western Bank, Sheffield, S10 2TN, UK

    P. D. Hempstead, P. M. Harrison, P. J. Artymyuk & V. V. Barynin

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  1. S. V. Antonyuk
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  2. V. R. Melik-Adamyan
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  3. A. N. Popov
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  4. V. S. Lamzin
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  5. P. D. Hempstead
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  6. P. M. Harrison
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  7. P. J. Artymyuk
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  8. V. V. Barynin
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__________

Translated from Kristallografiya, Vol. 45, No. 1, 2000, pp. 111–122.

Original Russian Text Copyright © 2000 by Antonyuk, Melik-Adamyan, Popov, Lamzin, Hempstead, Harrison, Artymyuk, Barynin.

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Antonyuk, S.V., Melik-Adamyan, V.R., Popov, A.N. et al. Three-dimensional structure of the enzyme dimanganese catalase from Thermus Thermophilus at 1 Å resolution. Crystallogr. Rep. 45, 105–116 (2000). https://doi.org/10.1134/1.171145

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  • DOI: https://doi.org/10.1134/1.171145

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