Summary
Flower buds and anthers of the short-day plant Pharbitis nil were treated either with thermic shock (7 or 35°C) or osmotic/trophic shock (12% sucrose) for 24 h. Explants were transferred either to Murashige and Skoog medium (MS) with addition of 6-benzylaminopurine (BA; 4.4μM) and 6% sucrose or to the same growth medium containing 22 μM BA and 3% sucrose. Both media were supplemented with α-naphthaleneacetic acid (NAA; 0.55 μM). Osmotic/trophic shock stimulated the occurrence of shoots on flower buds grown on medium containing 22 μM BA. Thermic shock (7 and 35°C) inhibited this process on both types of explants. Regenerated plantlets were transferred to MS medium supplemented with 6% sucrose, gibberellic acid (GA3; 1.44μM), NAA (0.55 μM) and Ca2+ (0.66 mgl−1). After 3–4 wk they were able to produce flowers without photoperiodic induction.
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Trejgell, A., Wójciak, A. & Tretyn, A. Photoperiodically independent flowering of Pharbitis nil plants regenerated from flower buds. In Vitro Cell.Dev.Biol.-Plant 38, 564–568 (2002). https://doi.org/10.1079/IVP2002351
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DOI: https://doi.org/10.1079/IVP2002351