Summary
An efficient seed germination system was developed for an endemic and endangered orchid of the prairies of Bogotá, Colombia. The effects of three culture media [Murashige and Skoog (MS) salts (1962); Knudson C (KC) salts (1946); and Hydro-Coljap® salts], activated charcoal [0 and 0.5% (w/v)], α-naphthaleneacetic acid (NAA; 0.0, 2.68 and 5.37 μM), and four light regimes (white light, darkness, red light, and far-red light) on asymbiotic germination of Odontoglossum gloriosum, were studied. The best germination percentage and germination time was obtained on agar-solidified medium supplemented with Hydro-Coljap® salts and 2.68 μM NAA under red light with a 16-h photoperiod. The addition of activated charoal did not stimulate either germination or the development of O. gloriosum seedlings. This study describes a single medium-based protocol able to achieve more than 330 000 seedlings within 40wk, starting from a single capsule. This protocol is sufficient for both large-scale propagation and in vitro conservation of this threatened orchid.
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Bowles, M.; Jacobs, K.; Zettler, L.; Delaney, T. Crossing effects on seed viability and experimental germination of the federal threatened Platanthera leucophoea (orchidaceae). Rodora 104:14–30; 2002.
Casal, J. Fotomorfogénesis: la luz como factor regulador del crecimiento. In: Azcon, J.; Talon, M., eds. Fundamentos de fisiología vegetal. Madrid: McGraw-Hill; 2000:377–388.
Chan, C.; Chang, W. C. Micropropagation of Cymbidium ensifolium var. Misericors through callus-derived rhizomes. In Vitro Cell. Dev. Biol. Plant 36:517–520; 2000.
Chen, J.; Chang, W. Induction of repetitive embryogenesis from seed-derived protocorms of Phalaenopsis amabilis var. formosa shimadzu. In Vitro Cell. Dev. Biol. Plant 40:290–293; 2004.
Chen, L. A simple tissue culture method developed to accelerate growth of plant tissues. J. Prim. Ind. 6(2):116–123; 1978.
Chen, L.; Pan, R.; Chen, R. Effects of media, growth regulators and dividing on the growth of Cymbidium sinense protocorm cultured in vitro. J. Trop. Subtrop. Bot. 7:59–64; 1999.
Chen, T.; Cheng, J. T.; Chang, W. Multiple shoot formation and plant regeneration from stem nodal explants of Paphiopedilum orchids. In Vitro Cell. Dev. Biol. Plant 38:595–597; 2002.
Cordonnier, M. Monoclonal antibodies: molecular probes for the study of phytochrome. Photochem. Photobiol. 49:821–831; 1989.
Gómez, K. R. Cultivo de células y tejidos. In: Pérez Ponce, J. N., ed. Propagación y mejora genética de plantas por biotecnología. Cuba: Instituto de biotecnología de las plantas; 1998:32.
González, A. Do 1: Econtro Nacional de orquidófilos pela. Copacabana Place, Rio de Janeiro, Brazil: Sociedad Brasileira de Orquidófilos (SBO); 1980.
Grillo, M.; Peña, E.; Pérez, D. Germinación masiva in vitro de algunas especies de orquídeas en el Jardín Botánico Nacional. Revista del Jardín Botánico Nacional (Universidad de la Habana) 6(2):95–100; 1985.
Hartman, H.; Kester, D.; Davies, F.; Geneve, R. Plant propagation: principles and practices, 6th edn. New Jersey: Prentice-Hall; 1997:192.
Instituto Alexander von Humbolt. Informe nacional sobre el estado de biodiversidad: especies de plantas superiores amenazadas. Columbia: Instituto Alexander von Humbolt; 1997.
Knudson, C. A new nutrient solution for the germination of orchid seeds. Am. Orchid. Soc. Bull. 14:214–217; 1946.
Krikorian, A. D. Propagación clonal in vitro. In: Roca, W.; Mroginski, L. A., eds. Cultivo de tejidos en la agricultura: fundamentos y applicaiones. Cali: CIAT; 1991:96–125.
Lee, Y. I.; Lee, N. Plant regeneration from protocorm-derived callus of Cypripedium formosanum. In Vitro Cell. Dev. Biol. Plant 39: 475–482; 2003.
Lin, Y. J.; Chen, C.; Yeh, F.; Chiu N.; Tsay, H. Tissue culture of Bletilla formosana. I. The influence of seed maturity and pretreatment on seed germination and seedling development. J. Agric. Res. China. 43:40–50; 1994.
Martin, K. Clonal propagation, encapsulation and reintroduction of Ispea malabarica (Reich. f.) J.D. Hook, an endangered orchid. In Vitro Cell. Dev. Biol. Plant 39:322–328; 2003.
Martínez, A. P.; Rubluo, A. In vitro mass propagation and population recovery of endangered tropical orchids. In: Angarita, A., ed. International Congress of Plant Tissue Culture of Tropical Species (Abstracts), Bogotá, 1997:56.
Mohr, H. Plant physiology. Berlin: Springer-Verlag; 1995.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–479; 1962.
Murthy, H.; Pyati, A. Micropropagation of Aerides maculosum Lindl. (Orchidaceae). In Vitro Cell. Dev. Biol. Plant 5:223–226; 2001.
Ortiz, P. Las orquídeas en Colombia. Pérez-Arbelaezia 5(11):72–73; 2000.
Park, S. Y.; Murthy, H. N.; Paek, K. Y. Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In Vitro Cell. Dev. Biol. Plant 38:168–172; 2002.
Pedroza, J.; Corchuelo, G.; Angarita, A. Control de la vitrificación en la micropropagación de estatice (Limonium sinuatum Mill cv. Midnight blue). Agronomía Colombiana 14(1):1–12; 1997.
Pedroza, J.; Fernández, C.; Suárez, A Evaluation of the effect of three growth regulators in the germination of Comparettia falcata seeds under in vitro conditions. J In Vitro Cell. Dev. Biol. Plant 41:838–843; 2005.
Peláez, J. M. Embriogénesis somática en Epidendrum ruizianum (Orchidaceae). Bogotá: Universidad Nacional de Colombia, Facultad de Ciencias, Departmento de Biología; 2002:40.
Pierik, R. L. M. Cultivo in vitro de las plantas superiores. Madrid: Mundi-Prensa; 1990.
Pyati, A.; Murthy, H.; Hahn, E.; Paek, K. In vitro propagation of Dendrobium macrostachyum Lindl.—a terrestrial threatened orchid. Indian J. Exp. Biol. 40:620–623; 2002.
Rivera, G. Orquídeas: generalidades y cultivo. Heredia: Efuna; 1998; 266 pp.
Rojas, M. Fisiología vegetal aplicada, 4th edn. México: McGraw-Hill; 1993.
Ruíz, G. Orquídeas, posible alternativa a cultivos ilícitos. Acopaflor 3(4):26–27; 1996.
Ruíz, M. C.; Orozco, M. Efectos de la luz y los reguladores del crecimiento sobre el porcentaje de germinación de Alnus acuminata H.B.K. (Betulaceae). Pérez-Arbelaezia 1(2):211–224; 1986.
Salisbury, F.; Ross, C. Fisiología Vegetal: Desarrollo de las plantas y fisiología ambiental. España: Thomson editores Paraninfo, S.A.; 2000:573–574.
So-Young, P.; Hosakatte, N.; Kee-Yoeup, P. Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In Vitro Cell. Dev. Biol. Plant 38:168–172; 2002.
Steel, R.; Torrie, J. Bioestadística: Principios y procedimientos, 2nd edn. Bogotá, McGraw Hill; 1985:328–367.
Takahashi, K.; Ogiwara, I.; Acoda, N. Seed germination of Habenaria radiata (Orchidaceae: Orthideae) in vitro. Lindleyana 15:59–63; 2000.
Vij, S. P.; Kondo, K.; Pathak, P. Gegeneration potential of Cymbidium pendulum (Roxb) Sw. nodal explants: a study in vitro. J. Orch. Soc. India 8:19–23; 1994.
Yates, R. C.; Curtis, J. T. The effect of sucrose and other factors on the shoot-root ratio of orchid seedlings. Am. J. Bot. 36:390–396; 1949.
Zettler, L.; Stewardt, S.; Bowles, M.; Jacobs, K. Mycorrhizal fungi and cold-assisted symbiotic germination of the federally threatened eastern prairie fringed orchid, Platanthera leucophaea (Nuttall) Lindley. Am. Midland Nat. 145:168–175; 2001.
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Pedroza-Manrique, J., Mican-Gutiérrez, Y. Asymbiotic germination of Odontoglossum gloriosum RCHB.F (Orchidaceae) under in vitro conditions. In Vitro Cell.Dev.Biol.-Plant 42, 543–547 (2006). https://doi.org/10.1079/IVP2006793
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DOI: https://doi.org/10.1079/IVP2006793