Improvement of Agrobacterium-mediated transformation of embryogenic calluses from maize elite inbred lines
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
This study was carried out to evaluate the effects of purine synthesis inhibitor mizoribine, purine and pyrimidine synthesis inhibitors azaserine and acivicin, and surfactant Silwet L-77 on Agrobacterium-mediated transformation efficiency of embryogenic calluses from maize elite inbred lines Qi 319 and Ye 515. After transformation and three rounds of selection on 2.8 μM chlorsulfuron, resistant calluses were obtained subsequently, and morphologically normal plantlets were regenerated from 80 to 90% of the resistant calluses treated with the compounds. There were no obvious discrepancies between the frequencies of plantlet regeneration and the ratio of PCR positive plantlets of calluses treated with different compounds. Results of PCR assay with primers for betA showed that 40.2% (103/256) of the regenerated plantlets were positive. The percentage of resistant calluses was 2–3-fold higher than the control after being treated with 0.19–0.27 mM mizoribine. The most suitable concentration of azaserin was 0.36 mM, which gave a 4-fold increase in the percentage of resistant calluses. Acivicin at 0.28–0.84 mM yielded a 3–5-fold increase in the percentage of resistant calluses, which is significantly better than the control. When the calluses were treated with 0.01 or 0.02% Silwet L-77, the percentages of resistant calluses were 34.89 and 25.60%, respectively. We concluded that purine synthesis inhibitors, purine and pyrimidine synthesis inhibitor and the surfactant Silwet L-77 at optimal concentrations significantly improved the Agrobacterium-mediated transformation of maize calluses.
- Boulton, M. I.; Buchholz, W. G.; Marks, M. S.; Markham, P. G.; Davies, J. W. Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae. Plant Mol. Biol. 12:31–40; 1989. CrossRef
- Cheng, M.; Fry, J. E.; Pang, S. Z.; Zhou, H. P.; Hironaka, C. M.; Duncan, D. R.; Conner, T. W.; Wan, Y. C. Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol. 115:971–980; 1997.
- Clough, S. J.; Bent, A. F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16:735–743; 1998. CrossRef
- Frame, B. R.; Shou, H.; Chikwamba, R. K.; Zhang, Z.; Xiang, C.; Fonger, T. M.; Pegg, S. E.; Li, B.; Nettleton, D. S.; Pei, D.; Wang, K. Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system. Plant Physiol. 129(1):13–22; 2002. CrossRef
- Grimsley, N.; Hohn, T.; Davis, J. W.; Hohn, B. Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325:177–179; 1987. CrossRef
- Hansen, G.; Wright, M. S. Recent advances in the transformation of plants. Trends Plant Sci. 4:226–231; 1999. CrossRef
- Huang, L.; Wei, Z. M. Agrobacterium tumefaciens mediated maize transformation. Acta Biol. Exp. Sin. 32(4):381–389; 1999(in Chinese).
- Ishida, Y.; Saito, H.; Ohta, S.; Hiei, Y.; Komari, T.; Kumashiro, T. High efficiency transformation of maize mediated by Agrobacterium tumefaciens. Nat. Biotechnol. 14:745–750; 1996. CrossRef
- Li, S. R.; Zhang, J. R.; Chen, H. M. Study on induction of embryogenic callus and plantlet regeneration in maize. J. Shandong Univ. 25(1):116–124; 1990 (in Chinese).
- Quan, R. D.; Shang, M.; Zhang, J. R. Optimization of transformation conditions in maize inbred line calluses via Agrobacterium tumefaciens. J. Plant Physiol. Mol. Biol. 29(3):245–250; 2003 (in Chinese).
- Quan, R. D.; Shang, M.; Zhang, J. R. Improved chilling tolerance by transformation with betA gene for the enhancement of glycinebetaine synthesis in maize. Plant Sci. 166:141–149; 2004. CrossRef
- Roberts, R. L.; Metz, M.; Monks, D. E.; Mullaney, M. L.; Hall, T.; Nester, E. W. Purine synthesis and increased Agrobacterium tumefaciens transformation of yeast and plants. Proc. Natl. Acad. Sci. USA 100(11):6634–6639; 2003. CrossRef
- Wu, H.; Sparks, C.; Amoah, B.; Jones, H. D. Factors influencing successful Agrobacterium-mediated genetic transformation of wheat. Plant Cell Rep. 21:659–668; 2003.
- Zhao, Z. Y.; Gu, W.; Tagliani, L.; Hondred, D.; Bond, D.; Schroeder, S.; Rudert, M.; Pierce, D. High throughout genetic transformation mediated by Agrobacterium tumefaciens in maize. Mol. Breed. 8(4): 323–333, 2002. CrossRef
- Improvement of Agrobacterium-mediated transformation of embryogenic calluses from maize elite inbred lines
In Vitro Cellular & Developmental Biology - Plant
Volume 42, Issue 3 , pp 215-219
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- purine synthesis inhibitor
- surfactant Silwet L-77
- transformation efficiency
- Industry Sectors