Summary
Valeriana glechomifolia is an endemic species of southern Brazil, capable of accumulating, in all of its organs, the terpene derivatives known as valepotriates, the presumed sedative components of the roots of pharmaceutically used species of Valeriana. In vitro cultures of the plant were established and the accumulation of acevaltrate, didrovaltrate, and valtrate in callus, cell suspension, and untransformed root cultures was studied. Leaves of in natura plants and roots of micropropagated plantlets were used as the explants for callus induction and root culture establishment, respectively, on Gamborg B5 basal medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or with kinetin (KIN). Culture growth and secondary metabolite yields were enhanced with 2,4-D (4.52μM) and KIN (0.93μM). Maximum valepotriate contents, quantified by HPLC, of acevaltrate (ACE) 2.6mg g−1 DW, valtrate (VAL) 10.2mgg−1 DW, and didrovaltrate (DID) 2.9mg g−1 DW were observed in root cultures after 7–8wk of culture.
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Backlund, A.; Moritz, T. Phylogenetic implications of an expanded valepotriate distribution in the Valerianaceae. Biochem. Syst. Ecol. 26:309–335; 1998.
Banerjee, S.; Rahman, L.; Uniyal, G. C.; Ahuja, P.S. Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy roots cultures of Valeriana wallichii DC. Plant Sci. 131:203–208; 1998.
Becker, H.; Chavadej, S. Valepotriates production by plant cell cultures. In: Bajaj, Y. P. S. ed. Biotechnology in agriculture and forestry, vol. 49. Berlin: Springer-Verlag; 1988:294–307.
Becker, H.; Schrall, R. Valepotriates in tissue cultures of nine different Valerianaceae species in comparison to literature data of the intact plants. J. Nat. Prod. 43:721–723; 1980.
Caetano, L. C.; Charlwood, B. V.; Gahan, P. B. The localization and accumulation of valepotriates in hairy roots cultures of Valerianella discoidea (L.) Loisel. Phytochem. Anal. 10:181–186; 1999.
De Carvalho, C. M. B.; Maurmann, N.; Luz, D. I.; Fett-Neto, A. G.; Rech, S. B. Control of development and valepotriate production by auxins in micropropagated Valeriana glechomifolia. Plant Cell Rep. 23:251–255; 2004.
Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158; 1968.
Gränicher, F.; Christen, P.; Kapetanidis, I. High yield production of valepotriates by hairy root cultures of Valeriana officinalis var. sambucifolia Mikan. Plant Cell Rep. 11:339–342; 1992.
Herrera-Arellano, A.; Luna-Villegas, G.; Cuevas-Uriostegui, M. L.; Alvarez, L.; Vargas-Pineda, G.; Zamilpa-Alvarez, A.; Tortoriello, J. Polysomnographic evaluation of the hypnotic effect of Valeriana edulis standardized extract in patients suffering from insomnia. Planta Med. 67:695–699; 2001.
Houghton, P. J. The scientific basis for the reputed activity of Valerian. J. Pharm. Pharmacol. 51:505–512; 1999.
Kittipongpatana, N.; Davis, D. L.; Porter, J. R. Methyl jasmonate increases the production of valepotriates by transformed root cultures of Valerianella locusta. Plant Cell Tiss. Organ Cult. 71:65–75; 2002.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.
Pras, N. The encyclopedia of cell technology. New York: Wiley; 2000.
Salles, L. A. Estudo fitoquímico e estabelecimento de culturas de calos de Valeriana glechomifolia Meyer. MSc thesis, Pharmacy School, Federal University of Rio Grande do Sul, Brazil; 1999.
Salles, L. A.; Silva, A. L.; Fett-Neto, A. G.; von Poser, G. L.; Rech, S. B. Valeriana glechomifolia: in vitro propagation and production of valepotriates. Plant Sci. 163:165–168; 2002.
Salles, L. A.; Silva, A. L.; Rech, S. B.; Zanatta, N.; von Poser, G. L. Constituents of Valeriana glechomifolia Meyer. Biochem. Syst. Ecol. 28:907–910; 2000.
Silva, A. L.; Rech, S. B.; von Poser, G. L. Quantitative determination of valepotriates from Valeriana native to south Brazil. Planta Med. 68:570–572; 2002.
Sobral, M. Flora ilustrada do rio grande do sul: valerianacea. Bol. Inst. Biociên. 58:1–61; 1999.
Sokal, R. R.; Rohlf, F. J. Biometry, San Francisco: W.H. Freeman; 1981.
Violon, C.; Dekegel, D.; Vercruysse, A. Relation between valepotriate content and differentiation level in various tissues from Valerianeae. J. Nat. Prod. 47:934–940; 1984.
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Maurmann, N., De Carvalho, C.M.B., Silva, A.L. et al. Valepotriates accumulation in callus, suspended cells and untransformed root cultures of Valeriana glechomifolia . In Vitro Cell.Dev.Biol.-Plant 42, 50–53 (2006). https://doi.org/10.1079/IVP2005725
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DOI: https://doi.org/10.1079/IVP2005725