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Evaluation of the effect of three growth regulators in the germination of comparettia falcata seeds under in vitro conditions

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Summary

The effect of 3-indoleacetic acid (IAA), 6-furfurylaminopurine (kinetin), and gibberellic acid (GA3) on germination of the orchid Comparettia falcata was evaluated in a factorial experiment (4×4×4) with Murashige and Skoog (1962) basal medium. It was established that seeds of this orchid could be maintained under aseptic conditions as long as the necessary nutrients and appropriate concentrations of growth regulators were provided. Of the three growth regulators used, IAA significantly decreased seed germination of Comparettia falcata. There was a synergistic effect in the kinetin:GA3 combination that produced a positive response in both percentage seed germination and development of seedlings. This study describes a single medium-based protocol able to achieve more than 160000 seedlings within 21 wk, starting from a single capsule, sufficient for both large-scale propagation and in vitro conservation of this threatened orchid.

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References

  • Arditti, J.; Ernst, R. Micropropagation of orchilds. New York: John Wiley; 1993.

    Google Scholar 

  • Bowles, M.; Jacobs, K.; Zettler, L.; Delaney, T. Crossing effects on seed viability and experimental germination of the federal threatened Platanthera leucophoea (Orchidaceae). Rodora 104:14–30; 2002.

    Google Scholar 

  • Chan, C.; Chang, W. C. Micropropagation of Cymbidium ensifolium var. Misericors through callus-derived rhizomes. In Vitro Cell. Dev. Biol. Plant. 36:517–520; 2000.

    Article  Google Scholar 

  • Chen, T.; Cheng, J. T.; Chang, W. Multiple shoot formation and plant regeneration from stem nodal explants of Paphiopedilum orchids. In Vitro Cell. Dev. Biol. Plant 38:595–597; 2002.

    CAS  Google Scholar 

  • Datta, K.; Kanjilal, B.; De Sarker, D. Artificial seed technology development of a protocol in Geodorum densifiorum (Lam) Scltr., an endangered orchid. Curr. Sci. 76:1142–1145; 1999.

    Google Scholar 

  • de Wilde, A. Orquídeas silvestres de Ucumarí. Colombia: Corporación autónoma de risaralda, 1994; 27 p.

    Google Scholar 

  • Dixon, K. Raising terrestrial orchids from seed. In: Harris, W. K., ed. Modern orchid growing for pleasure and profit. Adelaide: Orchid Club of South Australia, Inc.; 1987:47–100.

    Google Scholar 

  • Garcidueñas, M.; Ramírez, H. Control hormonal del desarrollo de la planta: Fisiología tecnología-experimentación, 2nd edn. México: Limusa; 1993:105–111.

    Google Scholar 

  • Gil-Martínez, F. Elementos de fisiología vegetal. España: Mundi-Prensa; 1995:1147.

    Google Scholar 

  • Hartman, H.; Kester, D.; Davies, F.; Geneve, R. Plant propagation: principles and practices, 6th edn. New Jersey: Prentice-Hall; 1997:125–144.

    Google Scholar 

  • Instituto Alexander von Humbolt. Informe nacional sobre el estado de biodiversidad: especies de plantas superiores amenazadas. Colombia: Alexander van Humbolt Institute; 1997.

    Google Scholar 

  • Jann, R.; Amen, R. What's germination?. In: Khan, A., ed. The physiology and biochemistry of seed dormancy and germination. Amsterdam: North Holland Publishing Company; 1980:7–26.

    Google Scholar 

  • Jones, R.; Stoddart, J. Gibberellins and seeds germination. In: Khan, A., ed. The physiology and biochemistry of seed dormancy and germination. Amsterdam: North Holland Publishing Company; 1980:97–104.

    Google Scholar 

  • Khan, A. The physiology and biochemistry of seed dormancy and germination. Amsterdam: North Holland Publishing Company; 1980:125.

    Google Scholar 

  • Kinderen, V. D. Orchideen In: Pierik, R. L. M., ed. Cultivo in vitro de las plantas superiores. Madrid: Mundi-Prensa; 1990:154.

    Google Scholar 

  • Lee, Y. I.; Lee, N. Plant regeneration from protocorm-derived callus of Cypripedium formosanum. In Vitro Cell. Dev. Biol. Plant 39:475–482; 2003.

    Article  Google Scholar 

  • Martin, K. Clonal propagation, encapsulation and reintroduction of Ispea malabarica (Reich. f.) J.D. Hook, an endangered orchid. In Vitro Cell. Dev. Biol. Plant 39:322–328; 2003.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–479; 1962.

    Article  CAS  Google Scholar 

  • Murthy, H.; Pyati, A. Micropropagation of Aerides maculosum Lindl. (Orchidaceae). In Vitro Cell. Dev. Biol. Plant 37:223–226; 2001.

    CAS  Google Scholar 

  • Nayak, N.; Rath, S.; Patnaik, S. High frequency plant regeneration from alginate encapsulated protocorm-like bodies of Spatholottis plicata BI, a terrestrial orchid. Phytomorphology 48:179–186; 1998.

    Google Scholar 

  • Palni, L. M.; Burch, L. R.; Jorgan, R. Los efectos de la concentración de las auxinas en la estabilidad de la citoquinina y el metabolismo. In: Azcon-Bieto, J.; Talon, M., eds. Fisiología y bioquímica vegetal, 1st edn Madrid: Interamericana, McGraw-Hill; 1993:323.

    Google Scholar 

  • Park, S. Y.; Murthy, H. N.; Paek, K. Y. Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In Vitro Cell. Dev. Biol. 38:168–172; 2002.

    Article  Google Scholar 

  • Pierik, R. L. M. Cultivo in vitro de las plantas superiores. Madrid: Mundi-Prensa; 1990.

    Google Scholar 

  • Pyati, A.; Murthy, H.; Hahn, E.; Paek, K. In vitro propagation of Dendrobium macrostachyum Lindl.—A terrestrial threatened orchid. Indian J. Exp. Biol. 40:620–623; 2002.

    PubMed  CAS  Google Scholar 

  • Rasmussen, H. N. Terrestrial orchids: from seed to mycotrophic plant. Cambridge: Cambridge University Press; 1995.

    Google Scholar 

  • Salisbury, F.; Ross, C. Fisiología vegetal: desarrollo de las plantas y fisiología ambiental. España: Thomson editores Paraninfo, SA; 2000:573–574.

    Google Scholar 

  • Seeni, S.; Latha, P. Foliar regeneration of the endangered Red Vanda, Renanthera imschootiana. Plant Cell Tiss. Org. Cult. 29:167–172; 1994.

    Article  Google Scholar 

  • Sharma, J.; Zettler, L.; Sambeek, J.; Mark, R. Symbiotic seed germination and mycorrhizae of federally threatened Platanthera praeclara (Orchidadeae). Am. Midland Nat. 149:104–121; 2003.

    Article  Google Scholar 

  • Shimasaki, K.; Uemoto, S. Rhizome induction and plantlet regeneration of Cymbidium goeringii from flower bud cultures in vitro. Plant Cell Tiss. Org. Cult. 25:49–52; 1991.

    Article  CAS  Google Scholar 

  • Singh, F. Differential staining of orchid seeds for viability testing. Am. Orchid Soc. Bull. 50(4):416–418; 1981.

    Google Scholar 

  • Smith, S.; Read, D. Orchid mycorrhizas. In: Mycorrhizal symbiosis, 2nd edn. California: Academic Press; 1997.

    Google Scholar 

  • Steel, R.; Torrie, J. Bioestadística: Principios y procedimientos, 2nd edn. Bogotá: McGraw Hill; 1985:328–367.

    Google Scholar 

  • Taiz, L.; Zeiger, E. Plant physiology, 2nd edn. Sunderland, MA: Sinauer Associates, Inc.; 1998:557.

    Google Scholar 

  • Takahashi, K.; Ogiwara, I.; Acoda, N. Seed germination of Habenaria radiata (Orchidaceae: Orchideae) in vitro. Lindleyana 15:59–63; 2000.

    Google Scholar 

  • Tanaka, M. Micropropagation of Phalaenopsis spp. In: Bajaj, Y. P. S., ed. High-tech and micropropagation IV. Biotechnology in agriculture and forestry, vol. 20. New York: Springer; 1992:246–266.

    Google Scholar 

  • Thomas, T. H. Cytokinin-active compounds and seed germination. In: Khan, A. A., ed. The physiology and biochemistry of seed dormancy and germination. Amsterdam: North Holland Publishing Company; 1980:111–137.

    Google Scholar 

  • Tokuhara, K.; Mii, M. Highly efficient somatic embryogenesis from cell suspension cultures of Phalaenopsis orchids by adjusting carbohydrate source. In Vitro Cell. Dev. Biol. Plant 39:635–642; 2003.

    Article  Google Scholar 

  • Van Overbeek J. El control del crecimiento vegetal, 1970. In: Garcidueñas, M., ed. Fisiología vegetal aplicada, 4th edn. México: Interamericana McGraw Hill; 1993:208.

    Google Scholar 

  • Zettler, L.; Stewardt, S.; Bowles, M.; Jacobs, K. Mycorrhizal fungi and cold-assisted symbiotic germination of the federally threatened eastern prairie fringed orchid, Platanthera leucophaea (Nuttall) Lindley. Am. Midland Nat. 145:168–175; 2001.

    Article  Google Scholar 

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Authors and Affiliations

  1. Facultad de Ciencias y Educación, Universidad Distrital Francisco, José de Caldas, Bogotá D.C., Colombia

    Jaime Pedroza-Manrique, Christian Fernandez-Lizarazo & Angelica Suarez-Silva

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  1. Jaime Pedroza-Manrique
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  2. Christian Fernandez-Lizarazo
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  3. Angelica Suarez-Silva
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Correspondence to Jaime Pedroza-Manrique.

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Pedroza-Manrique, J., Fernandez-Lizarazo, C. & Suarez-Silva, A. Evaluation of the effect of three growth regulators in the germination of comparettia falcata seeds under in vitro conditions. In Vitro Cell.Dev.Biol.-Plant 41, 838–843 (2005). https://doi.org/10.1079/IVP2005698

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  • DOI: https://doi.org/10.1079/IVP2005698

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