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Effect of triacontanol on the micropropagation of Costus speciosus (Koen.) SM. Using rhizome thin sections

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Summary

The highest percentage of shoot regeneration of Costus speciosus was achieved using thin rhizome sections and triacontanol (TRIA). Factors affecting the rate of shoot multiplication and rooting with TRIA have been investigated. Initiation of shoot buds was observed when rhizome thin sections were cultured on B5 basal medium supplemented with 5μgl−1 TRIA. Shoots with two to three leaves produced roots when cultured on B5 basad medium supplemented with 2 μgl−1 TRIA. The well-rooted shoots were hardened and transferred to soil where they showed normal growth and a 100% survival rate was achieved. Results of this study showed that TRIA can be used as an effective growth regulator in the micropropagation and conservation of C. speciosus.

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Authors and Affiliations

  1. Division of Plant Biotechnology, Department of Botany, Karnatak University, Pavate Nagar, 580003, Karnataka State, Dharwad, India

    Ravindra B. Malabadi, Gangadhar S. Mulgund & K. Nataraja

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  1. Ravindra B. Malabadi
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  2. Gangadhar S. Mulgund
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  3. K. Nataraja
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Correspondence to Ravindra B. Malabadi.

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Malabadi, R.B., Mulgund, G.S. & Nataraja, K. Effect of triacontanol on the micropropagation of Costus speciosus (Koen.) SM. Using rhizome thin sections. In Vitro Cell.Dev.Biol.-Plant 41, 129–132 (2005). https://doi.org/10.1079/IVP2004586

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