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Microsatellite analysis of pollen flow in Rhododendron metternichii var. hondoense

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Ecological Research

Microsatellite analysis was used to characterize the patterns of pollen flow in a 150 m × 70 m quadrat containing 18 flowering trees of Rhododendron metternichii Sieb. et Zucc. var. hondoense Nakai. Using six microsatellite genetic markers and exclusion analysis, we determined the paternal trees of 216 seedlings germinated from five fruits produced by four adults. Each fruit was pollinated by a small number of largely adjacent trees, but 20–30% of pollen came from outside the quadrat. Adult trees that produced many flowers had a high self-pollination rate. A directional flow of pollen from late-blooming trees to early ones was observed.

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Author notes

  1. Yuji Isagi

    Present address: Graduate School for International Development and Cooperation, Hiroshima University, Higashi–Hiroshima, 739-8529, Japan

Authors and Affiliations

  1. Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima, 739-8529, Japan

    Yoshiaki Kameyama & Nobukazu Nakagoshi

  2. Laboratory of Silviculture, Kansai Research Center, Forestry and Forest Products Research Institute, Kyoto, 612-0855, Japan

    Yuji Isagi

  3. Rural Ecology and Wildlife Conservation Division, Institute of Natural and Environmental Sciences, Himeji Institute of Technology, Toyooka, 668-0814, Japan

    Kazuaki Naito

Authors
  1. Yoshiaki Kameyama
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  2. Yuji Isagi
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  3. Kazuaki Naito
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  4. Nobukazu Nakagoshi
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Correspondence to Yoshiaki Kameyama.

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Kameyama, Y., Isagi, Y., Naito, K. et al. Microsatellite analysis of pollen flow in Rhododendron metternichii var. hondoense. Ecol Res 15, 263–269 (2000). https://doi.org/10.1046/j.1440-1703.2000.00347.x

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  • DOI: https://doi.org/10.1046/j.1440-1703.2000.00347.x

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