Abstract
Psychostimulant use disorder and schizophrenia have a substantial genetic basis. Evidence from human and animal studies on the involvement of the γ-aminobutyric acid (GABA) system in methamphetamine (METH) use disorder and schizophrenia is mounting. As we tested for the association of the human GABAA receptor gamma 2 subunit gene (GABRG2) with each diagnostic group, we used a case–control design with a set of 178 subjects with METH use disorder, 288 schizophrenics and 288 controls. First, we screened 96 controls and identified six SNPs in GABRG2, three of whom we newly reported. Next, we selected two SNPs, 315C>T and 1128+99C>A, as representatives of the linkage disequilibrium blocks for further case–control association analysis. Although no associations were found in either allelic or genotypic frequencies, we detected a haplotypic association in GABRG2 with METH use disorder, but not with schizophrenia. This finding partly replicates a recent case–control study of GABRG2 in METH use disorder, and thus indicates that GABRG2 may be one of the susceptibility genes of METH use disorder.
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Abbreviations
- METH:
-
methamphetamine
- GABA:
-
γ-aminobutyric acid
- GABRG2:
-
The human GABAA receptor gamma 2 subunit gene
- GAD:
-
glutamic acid decarboxylase
- PFC:
-
prefrontal cortex
- LD:
-
linkage disequilibrium
- DHPLC:
-
denaturing high-performance liquid chromatography
- PCR-RFLP:
-
polymerase chain reaction-restriction fragment lengthpolymorphism
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Acknowledgements
We gratefully acknowledge the helpful discussions with Dr J Ohashi on several points in the paper. We thank Ms Y Zusho and Ms M Miyata for their technical support. This work was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology, and the Ministry of Health, Labor and Welfare.
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Nishiyama, T., Ikeda, M., Iwata, N. et al. Haplotype association between GABAA receptor γ2 subunit gene (GABRG2) and methamphetamine use disorder. Pharmacogenomics J 5, 89–95 (2005). https://doi.org/10.1038/sj.tpj.6500292
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DOI: https://doi.org/10.1038/sj.tpj.6500292
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