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Specific gene expression profiles distinguish among functional allelic variants of the mouse Pthlh gene in transfected human cancer cells

  • Oncogenomics
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Abstract

The mouse parathyroid hormone-like hormone (Pthlh) gene encodes three allelic variants characterized by amino acid substitutions that are associated with susceptibility (PthlhPro) or resistance (PthlhThr and PthlhSerAspTyr) to two-stage skin carcinogenesis and to modulation of cell migration in vitro in transfected human cancer cells. cDNA microarray hybridization analysis of 8473 transcript clones revealed a similar gene expression profile for the PthlhThr and PthlhSerAspTyr alleles but a distinct pattern for the PthlhPro allele, suggesting an association between a specific gene expression profile and biological function of the Pthlh alleles. Some of the genes modulated by the Pthlh alleles, e.g., ANXA1, CCL2, FN1 and TFF3, play a role in cell migration and may represent candidate targets for this Pthlh function. Our study demonstrates the potential usefulness of gene expression profiling of genetic variants for the functional characterization of candidate cancer modifier genes.

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References

  • Benelli R, Peissel B, Manenti G, Gariboldi M, Vanzetto C, Albini A et al. (2003). Oncogene 22: 7711–7715.

  • Cambien B, Pomeranz M, Millet MA, Rossi B, Schmid-Alliana A . (2001). Blood 97: 359–366.

  • De Cecco L, Marchionni L, Gariboldi M, Reid JF, Lagonigro MS, Caramuta S et al. (2004). Oncogene 23: 8171–8183.

  • Deans C, Wigmore S, Paterson-Brown S, Black J, Ross J, Fearon KC . (2005). Cancer 103: 1810–1818.

  • Dragani TA . (2003). Cancer Res 63: 3011–3018.

  • Duxbury MS, Matros E, Clancy T, Bailey G, Doff M, Zinner MJ et al. (2005). Ann Surg 241: 491–496.

  • Dysvik B, Jonassen I . (2001). Bioinformatics 17: 369–370.

  • Funk JL . (2001). Int J Immunopharmaco 1: 1101–1121.

  • Hiraki A, Ueoka H, Bessho A, Segawa Y, Takigawa N, Kiura K et al. (2002). Cancer 95: 1706–1713.

  • Hoffmann W, Jagla W, Wiede A . (2001). Histol Histopathol 16: 319–334.

  • Leeb SN, Vogl D, Grossmann J, Falk W, Scholmerich J, Rogler G et al. (2004). Am J Gastroenterol 99: 335–340.

  • Manenti G, Peissel B, Gariboldi M, Falvella FS, Zaffaroni D, Allaria B et al. (2000). Oncogene 19: 5324–5328.

  • Maus UA, Waelsch K, Kuziel WA, Delbeck T, Mack M, Blackwell TS et al. (2003). J Immunol 170: 3273–3278.

  • Nagase H, Mao JH, de Koning JP, Minami T, Balmain A . (2001). Cancer Res 61: 1305–1308.

  • Oertel M, Graness A, Thim L, Buhling F, Kalbacher H, Hoffmann W . (2001). Am J RespCell Mol 25: 418–424.

  • Parente L, Solito E . (2004). Inflamm Res 53: 125–132.

  • Ross DT, Scherf U, Eisen MB, Perou CM, Rees C, Spellman P et al. (2000). Nat Genet 24: 227–235.

  • Strewler GJ . (2000). N Engl J Med 342: 177–185.

  • Yio X, Zhang JY, Babyatsky M, Chen A, Lin J, Fan QX et al. (2005). Clin Exp Metastasis 22: 157–165.

  • Zhang ZW, Newcomb PV, Moorghen M, Gupta J, Feakins R, Savage P et al. (2004). Cancer Causes Control 15: 211–218.

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Acknowledgements

This work was funded in part by Grants from Associazione and Fondazione Italiana Ricerca Cancro (AIRC and FIRC), Italy and from EC RISC-RAD project.

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Authors and Affiliations

  1. Department of Experimental Oncology and Laboratories, Istituto Nazionale Tumori, Milan, Italy

    R Gianni-Barrera, M Gariboldi, L De Cecco, G Manenti & T A Dragani

  2. IFOM Foundation, Milan, Italy

    M Gariboldi & L De Cecco

Authors
  1. R Gianni-Barrera
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  2. M Gariboldi
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  3. L De Cecco
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  4. G Manenti
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  5. T A Dragani
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Correspondence to T A Dragani.

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Gianni-Barrera, R., Gariboldi, M., De Cecco, L. et al. Specific gene expression profiles distinguish among functional allelic variants of the mouse Pthlh gene in transfected human cancer cells. Oncogene 25, 4501–4504 (2006). https://doi.org/10.1038/sj.onc.1209478

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  • DOI: https://doi.org/10.1038/sj.onc.1209478

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