Abstract
It has been shown that methylation of CpG dinucleotides located in the promoter region of TP53 is associated with low expression levels of this gene. We have analysed the methylation status of one CpG dinucleotide and of three CCWGG motifs, also located in the promoter region of the gene, in bone marrow samples obtained from patients with acute lymphoblastic leukemia (ALL). Eight out of 25 samples analysed showed methylation of either the CpG dinucleotide, the CCWGG motifs or both. Relative to nonmethylated leukemia samples, TP53 expression levels were decreased in all methylated samples in which TP53 expression could be measured. Methylation of CpG and CCWGG motifs in the promoter of TP53 could represent a novel mechanism leading to functional impairment of this tumor suppressor gene in ALL.
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Acknowledgements
We are grateful to José P Román for his assistance with densitometric analyses. This work is supported in part by Grant 48/99 from the Department of Health of the Government of Navarra, Spain; and Grant 01/132 from the Fondo de Investigaciones Sanitarias; XA is supported by a grant from the Department of Education, Universities and Research of the Government of the Basque Country, Spain (BFI99.1.0AE).
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Agirre, X., Vizmanos, J., Calasanz, M. et al. Methylation of CpG dinucleotides and/or CCWGG motifs at the promoter of TP53 correlates with decreased gene expression in a subset of acute lymphoblastic leukemia patients. Oncogene 22, 1070–1072 (2003). https://doi.org/10.1038/sj.onc.1206236
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DOI: https://doi.org/10.1038/sj.onc.1206236
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