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Cell cycle regulated expression and phosphorylation of hpttg proto-oncogene product

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Abstract

We recently isolated a cDNA for hpttg, the human homolog of rat pituitary tumor transforming gene. Now we have analysed the expression of hpttg as a function of cell proliferation. hPTTG protein level is up-regulated in rapidly proliferating cells, is down-regulated in response to serum starvation or cell confluence, and is regulated in a cell cycle-dependent manner, peaking in mitosis. In addition, we show that hPTTG is phosphorylated during mitosis. Immunodepletion and in vitro phosphorylation experiments, together with the use of a specific inhibitor, indicate that Cdc2 is the kinase that phosphorylates hPTTG. These results suggest that hpttg is induced by, and may have a role in, regulatory pathways involved in the control of cell proliferation.

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Acknowledgements

We thank Dr RM Rios for useful discussions and suggestions and Drs S Gisselbrecht and S Fischer for critical reading of the manuscript. This work was supported by grants from Ministerio de Educación y Ciencia of Spain (SAF96-0275 and SAF99-0125), from DGUI of the Junta de Andalucía, and from Rhône-Poulenc Rorer (Paris).

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Authors and Affiliations

  1. Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Apdo. 1095, 41080-Sevilla, Spain

    Francisco Ramos-Morales, África Domínguez, Francisco Romero, Rosa Luna & María Tortolero

  2. Instituto de Recursos Naturales y Agrobiología. Apdo. 1052. 41080-Sevilla, Spain

    Francisco Ramos-Morales & José A Pintor-Toro

  3. Rhône-Poulenc Rorer, CRVA-Pasteur Building, 13, quai J. Guesde. 94403 Vitry-sur-Seine, France

    Marie-Christine Multon

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  1. Francisco Ramos-Morales
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  2. África Domínguez
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Ramos-Morales, F., Domínguez, Á., Romero, F. et al. Cell cycle regulated expression and phosphorylation of hpttg proto-oncogene product. Oncogene 19, 403–409 (2000). https://doi.org/10.1038/sj.onc.1203320

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  • DOI: https://doi.org/10.1038/sj.onc.1203320

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