Abstract
The transcription factor Forkhead box protein P1 (FOXP1) is highly expressed in a proportion of diffuse large B-cell lymphoma (DLBCL). In this report, we provide cytogenetic and fluorescence in situ hybridization (FISH) data showing that FOXP1 (3p13) is recurrently targeted by chromosome translocations. The genomic rearrangement of FOXP1 was identified by FISH in three cases with a t(3;14)(p13;q32) involving the immunoglobulin heavy chain (IGH) locus, and in one case with a variant t(2;3) affecting sequences at 2q36. These aberrations were associated with strong expression of FOXP1 protein in tumor cells, as demonstrated by immunohistochemistry (IHC). The cases with t(3p13) were diagnosed as DLBCL ( × 1), gastric MALT lymphoma ( × 1) and B-cell non-Hodgkin's lymphoma, not otherwise specified ( × 2). Further IHC and FISH studies performed on 98 cases of DLBCL and 93 cases of extranodal marginal zone lymphoma showed a high expression of FOXP1 in approximately 13 and 12% of cases, respectively. None of these cases showed, however, FOXP1 rearrangements by FISH. However, over-representation of the FOXP1 locus found in one additional case of DLBCL may represent another potential mechanism underlying an increased expression of this gene.
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Acknowledgements
We thank AH Banham (John Radcliffe Hospital, University of Oxford, Oxford, UK) for providing the JC12 antibody, U Pluys and S Karanfil for technical assistance in FISH and IHC analysis, respectively, R Schots (AZ-VUB Jette, Brussels) and V Madoe (St Salvatorziekenhuis, Hasselt) for clinical data and R Logist for clerical help.
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This text presents research results of the Belgian program of Interuniversity Poles of attraction initiated by the Belgian State, Prime Minister's Office, Science Policy Programming. The scientific responsibility is assumed by the authors. P Vandenberghe is a Clinical Investigator of FWO-Vlaanderen
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Wlodarska, I., Veyt, E., De Paepe, P. et al. FOXP1, a gene highly expressed in a subset of diffuse large B-cell lymphoma, is recurrently targeted by genomic aberrations. Leukemia 19, 1299–1305 (2005). https://doi.org/10.1038/sj.leu.2403813
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DOI: https://doi.org/10.1038/sj.leu.2403813
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