Using a model system, the activities of α-L-arabinofuranosidase, β-glucosidase, and α-L-rhamonopyranosidase were determined in 32 strains of yeasts belonging to the genera Aureobasidium, Candida, Cryptococcus, Hanseniaspora, Hansenula, Kloeckera, Metschnikowia, Pichia, Saccharomyces, Torulaspora and Brettanomyces (10 strains); and seven strains of the bacterium Leuconostoc oenos. Only one Saccharomyces strain exhibited β-glucosidase activity, but several non-Saccharomyces yeast species showed activity of this enzyme. Aureobasidium pullulans hydrolyzed α-L-arabinofuranoside, β-glucoside, and α-L-rhamnopyranoside. Eight Brettanomyces strains had β-glucosidase activity. Location of enzyme activity was determined for those species with enzymatic activity. The majority of β-glucosidase activity was located in the whole cell fraction, with smaller amounts found in permeabilized cells and released into the growth medium. Aureobasidium pullulans hydrolyzed glycosides found in grapes.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received 02 February 1999/ Accepted in revised form 26 June 1999
Rights and permissions
About this article
Cite this article
McMahon, H., Zoecklein, B., Fugelsang, K. et al. Quantification of glycosidase activities in selected yeasts and lactic acid bacteria. J Ind Microbiol Biotech 23, 198–203 (1999). https://doi.org/10.1038/sj.jim.2900720
Issue Date:
DOI: https://doi.org/10.1038/sj.jim.2900720