Journal of Industrial Microbiology and Biotechnology

, Volume 19, Issue 4, pp 280-285

Optimization of fermentation conditions for the production of ethylene-diamine-disuccinic acid by Amycolatopsis orientalis

  • N ZwickerAffiliated withUniversität Tübingen, Biologisches Institut, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
  • , U TheobaldAffiliated withUniversität Tübingen, Biologisches Institut, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
  • , H ZähnerAffiliated withUniversität Tübingen, Biologisches Institut, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
  • , H-P FiedlerAffiliated withUniversität Tübingen, Biologisches Institut, Auf der Morgenstelle 28, D-72076 Tübingen, Germany

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The production of EDDS (ethylene-diamine-disuccinic acid), a potential substitute for EDTA, has been optimized up to a product concentration of 20 grams per litre in fermentations of Amycolatopsis orientalis. Decisive steps for the increase in productivity were variation of the synthetic medium composition, investigation of the influence of metal ions on product formation, controlled feeding of carbon and nitrogen sources in fed-batch fermentations and improvement of the downstream processing steps.

Keywords: Amycolatopsis orientalis; EDDS; cation chelator; synthetic medium; fed-batch fermentation; metal ions