Journal of Industrial Microbiology and Biotechnology

, Volume 19, Issue 4, pp 280–285

Optimization of fermentation conditions for the production of ethylene-diamine-disuccinic acid by Amycolatopsis orientalis

  • N Zwicker
  • U Theobald
  • H Zähner
  • H-P Fiedler

DOI: 10.1038/sj.jim.2900458

Cite this article as:
Zwicker, N., Theobald, U., Zähner, H. et al. J Ind Microbiol Biotech (1997) 19: 280. doi:10.1038/sj.jim.2900458

The production of EDDS (ethylene-diamine-disuccinic acid), a potential substitute for EDTA, has been optimized up to a product concentration of 20 grams per litre in fermentations of Amycolatopsis orientalis. Decisive steps for the increase in productivity were variation of the synthetic medium composition, investigation of the influence of metal ions on product formation, controlled feeding of carbon and nitrogen sources in fed-batch fermentations and improvement of the downstream processing steps.

Keywords: Amycolatopsis orientalis; EDDS; cation chelator; synthetic medium; fed-batch fermentation; metal ions

Copyright information

© Society for Industrial Microbiology 1997

Authors and Affiliations

  • N Zwicker
    • 1
  • U Theobald
    • 1
  • H Zähner
    • 1
  • H-P Fiedler
    • 1
  1. 1.Universität Tübingen, Biologisches Institut, Auf der Morgenstelle 28, D-72076 Tübingen, GermanyDE