Abstract
Linking proteins directly to nucleic acids has been a complex task. By hybridizing a bifunctional peptide nucleic acid (PNA) consisting of a nucleic acid binding moiety and a nuclear localization signal (NLS) we have previously demonstrated that it is possible to link protein functions directly to nucleic acids containing a PNA target site. By hybridizing fluorescently labeled oligonucleotides to PNA-NLS molecules and subsequently transfecting different organs in vivo we demonstrate an active nuclear translocation of the PNA-NLS/oligonucleotide complex in different mouse organs.
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This research has been supported by the Swedish Research Council.
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Brandén, L., Christensson, B. & Smith, C. In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides. Gene Ther 8, 84–87 (2001). https://doi.org/10.1038/sj.gt.3301345
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DOI: https://doi.org/10.1038/sj.gt.3301345
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