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A novel PCR-RFLP assay for the detection of a polymorphism in the 3′ of STAT6 gene

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Abstract

We have identified a novel single nucleotide polymorphism at position 2964 (G/A) in the 3′ untranslated region of the human STAT6 gene (GeneBank Accession No. U16031). A novel PCR-RFLP method has been devised for this polymorphism using the amplification-created restriction site (ACRS) method. Allele frequencies in the UK Caucasian population were found to be 0.33 for allele A and 0.67 for allele G.

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Authors and Affiliations

  1. ARC Epidemiology Unit, Manchester Medical School, Manchester University, Oxford Road, Manchester, M13 9PT, UK

    M Amoli, WER Ollier & AH Hajeer

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  1. M Amoli
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  2. WER Ollier
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  3. AH Hajeer
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Correspondence to AH Hajeer.

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Amoli, M., Ollier, W. & Hajeer, A. A novel PCR-RFLP assay for the detection of a polymorphism in the 3′ of STAT6 gene. Genes Immun 1, 349–350 (2000). https://doi.org/10.1038/sj.gene.6363684

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  • DOI: https://doi.org/10.1038/sj.gene.6363684

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