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Immunotherapy

Murine B-cell leukemia lymphoma (BCL1) cells as a target for NK cell-mediated immunotherapy

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Natural killer (NK) cells are important for their ability to recognize and lyse tumor cells and virus infected cells. NK cells express triggering receptors that are specific for non-MHC ligands. This article describes the 35S release cytotoxic assay, which measures the ability of NK cells derived from spleen cells taken from polyIC-treated mice to lyse B-cell leukemia (BCL1) cells. BCL1 cells express ligands for NKp46 on the cell surface membrane and they are sensitive to allogeneic but not syngeneic IL-2 activated natural killer cells.

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Acknowledgements

We thank the Danny Cunniff Leukemia Research Laboratory; the Gabrielle Rich Leukemia Research Foundation; the Cancer Treatment Research Foundation; the Novotny Trust and the Fig Tree Foundation for their continuous support of our ongoing basic and clinical research.

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Authors and Affiliations

  1. Department of Bone Marrow Transplantation and Cancer Immunotherapy, Cell Therapy and Transplantation Immunobiology Research Center, Hadassah University Hospital, Jerusalem, Israel

    L Weiss, S Reich & S Slavin

  2. The Lautenberg Center for General and Tumor Immunology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

    O Mandelboim

Authors
  1. L Weiss
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  2. S Reich
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  3. O Mandelboim
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  4. S Slavin
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Correspondence to S Slavin.

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Weiss, L., Reich, S., Mandelboim, O. et al. Murine B-cell leukemia lymphoma (BCL1) cells as a target for NK cell-mediated immunotherapy. Bone Marrow Transplant 33, 1137–1141 (2004). https://doi.org/10.1038/sj.bmt.1704475

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  • DOI: https://doi.org/10.1038/sj.bmt.1704475

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