A comparative study on the efficacy of CD8-positive cells in enhancing allogeneic bone marrow engraftment: cell sorting vs microbead selection

Abstract

We have used a superparamagnetic microbead selection system to positively select a murine bone marrow CD8⁺ cell population. The functional ability of these cells to enhance allogeneic bone marrow engraftment was compared with that of fluorescence activated cell sorter purified CD8⁺ cells. The CD8⁺ cell population prepared by the microbead selection procedure was as effective as cell sorter purified CD8⁺ cells in enhancing T cell-depleted allogeneic bone marrow engraftment in lethally irradiated mice. Phenotypic characterization of these cells shows that most of these CD8⁺ cells express CD3 and the T cell antigen receptor complex.