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Dissecting the cellular states and fates of shed mouse intestinal cells

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Analysis of cells shed from the mouse gut, using bulk and single-cell transcriptomics, as well as single-molecule FISH and intravital imaging, revealed that shed cells are diverse, remain viable for a few hours and upregulate anti-microbial gene expression programs.

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Fig. 1: Host transcriptomics of shed intestinal cells.

References

  1. Sender, R. & Milo, R. The distribution of cellular turnover in the human body. Nat. Med. 27, 45–48 (2021). This review describes the cellular turnover of cells throughout the human body.

    Article  CAS  PubMed  Google Scholar 

  2. Moor, A. E. et al. Spatial reconstruction of single enterocytes uncovers broad zonation along the intestinal villus axis. Cell 175, 1156–1167 (2018). This paper demonstrates that enterocytes upregulate the expression of hundreds of genes at the villi tips.

    Article  CAS  PubMed  Google Scholar 

  3. Manco, R. et al. Clump sequencing exposes the spatial expression programs of intestinal secretory cells. Nat. Commun. 12, 3074 (2021). This paper demonstrates that intestinal secretory cells also upregulate the expression of many genes at the villi tips.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Ungar, B. et al. Host transcriptome signatures in human faecal-washes predict histological remission in patients with IBD. Gut 71, 1988–1997 (2022). This paper demonstrates that bulk transcriptomics of faecal washes can robustly identify active inflammation in patients with IBD.

    Article  CAS  Google Scholar 

  5. Dan, S. et al. Distal fecal wash host transcriptomics identifies inflammation throughout the colon and terminal ileum. Cell Mol. Gastroenterol. Hepatol. 16, 1–15 (2023). This paper demonstrates that bulk transcriptomics of distal gut faecal washes can identify active inflammation in ileal Crohn’s disease, in line with the stability of shed cells demonstrated in our study.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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This is a summary of: Halpern, B. K. et al. The cellular states and fates of shed intestinal cells. Nat. Metab. https://doi.org/10.1038/s42255-023-00905-9 (2023).

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Dissecting the cellular states and fates of shed mouse intestinal cells. Nat Metab 5, 1854–1855 (2023). https://doi.org/10.1038/s42255-023-00907-7

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