Mammalian cell culture represents a cornerstone of modern biomedical research. There is growing appreciation that the media conditions in which cells are cultured can profoundly influence the observed biology and reproducibility. Here, we consider a key but often ignored variable, oxygen, and review why being mindful of this environmental parameter is so important in the design and interpretation of cell culture studies.
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V.K.M. is on the scientific advisory boards of Raze Therapeutics, Janssen Pharmaceuticals and 5AM Ventures, and is listed as an inventor on patents filed by Massachusetts General Hospital on the use of hypoxia as a therapy.
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Ast, T., Mootha, V.K. Oxygen and mammalian cell culture: are we repeating the experiment of Dr. Ox?. Nat Metab 1, 858–860 (2019). https://doi.org/10.1038/s42255-019-0105-0
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DOI: https://doi.org/10.1038/s42255-019-0105-0
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