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How to meet the need for speed in protein evolution

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Conventional experiments for generating proteins with improved properties by directed evolution are iterative, lengthy and costly. Now, a label-free assay has been developed for ultrahigh-throughput microfluidic screening that can dramatically accelerate the discovery of superior biocatalysts from a single round of genetic randomization.

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Fig. 1: Strategies used for activity detection in the engineering of CHAO for application in cyclic deracemization.

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  1. Clemens-Schöpf-Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany

    Wolf-Dieter Fessner

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  1. Wolf-Dieter Fessner
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Correspondence to Wolf-Dieter Fessner.

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Fessner, WD. How to meet the need for speed in protein evolution. Nat Catal 2, 738–739 (2019). https://doi.org/10.1038/s41929-019-0342-3

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