Single-molecule live-cell imaging of the transcription dynamics in budding yeast cells revealed that the remodeling of different nucleosomes in the promoter of a gene regulates various kinetic steps of transcription. The measurements also showed that the TATA-binding protein competes with promoter-associated nucleosomes around the TATA element to activate transcription.
References
Symmons, O. & Raj, A. What’s luck got to do with it: single cells, multiple fates, and biological nondeterminism. Mol. Cell 62, 788–802 (2016). A review describing several consequences of stochastic gene expression heterogeneity.
Brouwer, I. & Lenstra, T. L. Visualizing transcription: key to understanding gene expression dynamics. Curr. Opin. Chem. Biol. 51, 122–129 (2019). A review article that presents the results of imaging-based approaches to studying transcriptional bursting.
Brown, C. R., Mao, C., Falkovskaia, E., Jurica, M. S. & Boeger, H. Linking stochastic fluctuations in chromatin structure and gene expression. PLoS Biol. 11, e1001621 (2013). This paper identifies a link between stochastic nucleosome positioning and gene expression.
Brouwer, I., Patel, H. P., Meeussen, J. V. W., Pomp, W. & Lenstra, T. L. Single-molecule fluorescence imaging in living Saccharomyces cerevisiae cells. STAR Protoc. 1, 100142 (2020). A protocol article that presents the method used in this study to image transcription dynamics.
Wang, H., Xiong, L. & Cramer, P. Structures and implications of TBP-nucleosome complexes. Proc. Natl Acad. Sci. USA 118, 1–7 (2021). This paper provides structural data that support our TBP–nucleosome competition model.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Brouwer, I., Kerklingh, E., van Leeuwen, F. & Lenstra, T. L. Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-023-00981-1 (2023).
Rights and permissions
About this article
Cite this article
Quantifying how promoter-associated nucleosomes regulate transcriptional bursting. Nat Struct Mol Biol 30, 577–578 (2023). https://doi.org/10.1038/s41594-023-00982-0
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41594-023-00982-0
- Springer Nature America, Inc.