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CRISPR–Cas systems

CRISPR–Cas has a new juggling act: interplay between nuclease and protease

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Craspase is newly identified type III CRISPR–Cas system with two major components: the nuclease Cas7-11, and the protease TPR-CHAT. Craspases perform a delicate balancing act between nuclease and protease activity to achieve immune tolerance and defense in bacteria, and show promise as highly regulatable genome-editing tools.

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Fig. 1: Mechanisms of type III CRISPR–Cas systems in prokaryotic immune responses.

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Acknowledgements

T.-M.F. was supported by the National Institutes of Health (NIH) National Institute of General Medical Sciences (grant 1R35GM147465) and A.D.R was supported by an NIH T32 (GM118291-05 and GM144293-01).

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Authors and Affiliations

  1. Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, USA

    Anthony D. Rish & Tian-Min Fu

  2. The Ohio State University Comprehensive Cancer Center, Columbus, OH, USA

    Anthony D. Rish & Tian-Min Fu

  3. Program of OSBP, The Ohio State University, Columbus, OH, USA

    Anthony D. Rish & Tian-Min Fu

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  1. Anthony D. Rish
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  2. Tian-Min Fu
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Correspondence to Tian-Min Fu.

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Rish, A.D., Fu, TM. CRISPR–Cas has a new juggling act: interplay between nuclease and protease. Nat Struct Mol Biol 30, 126–128 (2023). https://doi.org/10.1038/s41594-022-00917-1

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