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DNA REPLICATION

Focus and persistence: how Pol IV unblocks stalled DNA synthesis

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The mechanisms by which translesion DNA polymerases mediate DNA repair are incompletely understood. A new study shows that Escherichia coli DNA polymerase IV is concentrated at the sites of arrested DNA synthesis by an interaction with SSB, the major single-stranded DNA-binding protein, specifically at stalled but not ongoing replication forks.

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Fig. 1: The two-step recruitment of Pol IV mediated by binding of SSB to ssDNA at sites of stalled DNA synthesis.

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Acknowledgements

Work in the Sale laboratory is supported by a core grant to the LMB from the MRC (U105178808).

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  1. Division of Protein & Nucleic Acid Chemistry, MRC Laboratory of Molecular Biology, Cambridge, UK

    Julian E. Sale

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  1. Julian E. Sale
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Correspondence to Julian E. Sale.

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Sale, J.E. Focus and persistence: how Pol IV unblocks stalled DNA synthesis. Nat Struct Mol Biol 29, 846–847 (2022). https://doi.org/10.1038/s41594-022-00825-4

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