We used single-nucleus sequencing to generate an atlas of gene expression and chromatin accessibility in the amygdala of outbred rats with divergent cocaine addiction-like behaviors. The results implicated dysregulation of metabolic pathways and GABAergic transmission as molecular bases of susceptibility or resistance to addiction.
References
Koob, G. F. & Volkow, N. D. Neurobiology of addiction: a neurocircuitry analysis. Lancet Psychiatry 3, 760–773 (2016). A review of the neurocircuitries involved in addiction.
Solberg Woods, L. C. & Palmer, A. A. in Rat Genomics (eds. Hayman, G. et al.) 233–247 (Humana, 2019). A review of the use of heterogeneous stock (HS) rodents for fine mapping of complex traits in rodents.
Carrette, L. L. G. et al. The cocaine and oxycodone biobanks, two repositories from genetically diverse and behaviorally characterized rats for the study of addiction. eNeuro https://doi.org/10.1523/ENEURO.0033-21.2021 (2021). A paper presenting the behavioral characterization of HS rats using the addiction index and a model of extended access to cocaine intravenous self-administration.
Munro, D. et al. The regulatory landscape of multiple brain regions in outbred heterogeneous stock rats. Nucleic Acids Res. 50, 10882–10885 (2022). A paper that maps expression quantitative trait loci in HS rats.
Distler, M. G. et al. Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal. J. Clin. Invest. 122, 2306–2315 (2012). A paper establishing the role of methylglyoxal as a GABAA receptor agonist.
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This is a summary of: Zhou, J. L. et al. Single-nucleus genomics in outbred rats with divergent cocaine addiction-like behaviors reveals changes in gene amygdala GABAergic inhibition. Nat. Neurosci. https://doi.org/10.1038/s41593-023-01452-y (2023).
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A single-cell atlas of the rat amygdala reveals molecular signatures of cocaine addiction. Nat Neurosci 26, 1838–1839 (2023). https://doi.org/10.1038/s41593-023-01454-w
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DOI: https://doi.org/10.1038/s41593-023-01454-w
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