New techniques enable simultaneous optogenetic stimulation and calcium imaging from ensembles of tens of neurons in vivo. Improved opsins are localized to the cell body, minimizing spurious activation of the optically unresolvable neuropil. Two-photon light pulses are sculpted in space, time, and wavelength to efficiently target the desired cells.
This is a preview of subscription content, log in via an institution to check access.
References
Mardinly, A.R. et al. Nat. Neurosci. 21, https://doi.org/10.1038/s41593-018-0139-8 (2018).
Dal Maschio, M., Donovan, J. C., Helmbrecht, T. O. & Baier, H. Neuron 94, 774–789.e5 (2017).
Forli, A. et al. Cell Rep. 22, 3087–3098 (2018).
Yang, W., Carrillo-Reid, L., Bando, Y., Peterka, D. S. & Yuste, R. eLife 7, e32671 (2018).
Kubota, Y. et al. Sci. Rep. 1, 89 (2011).
Wu, C., Ivanova, E., Zhang, Y. & Pan, Z. H. PLoS One 8, e66332 (2013).
Shemesh, O. A. et al. Nat. Neurosci. 20, 1796–1806 (2017).
Klapoetke, N. C. et al. Nat. Methods 11, 338–346 (2014).
Podgorski, K. & Ranganathan, G. J. Neurophysiol. 116, 1012–1023 (2016).
Rickgauer, J. P. & Tank, D. W. Proc. Natl Acad. Sci. USA 106, 15025–15030 (2009).
Packer, A. M., Russell, L. E., Dalgleish, H. W. & Häusser, M. Nat. Methods 12, 140–146 (2015).
Rickgauer, J. P., Deisseroth, K. & Tank, D. W. Nat. Neurosci. 17, 1816–1824 (2014).
Ronzitti, E. et al. J. Opt. 19, 113001 (2017).
Pégard, N. C. et al. Nat. Commun. 8, 1228 (2017).
Adam, Y. et al. bioRxiv https://doi.org/10.1101/281618 (2018).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Cohen, A.E., Farhi, S.L. Sculpting light to reveal brain function. Nat Neurosci 21, 776–778 (2018). https://doi.org/10.1038/s41593-018-0158-5
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41593-018-0158-5
- Springer Nature America, Inc.