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ARTR-seq for determining RNA-binding protein target sites through in situ reverse transcription

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ARTR-seq uses antibody-guided in situ reverse transcription to efficiently and accurately identify RNA-binding protein target sites in as few as 20 cells, or in a formaldehyde-fixed tissue section. The high temporal resolution of ARTR-seq opens opportunities for the investigation of dynamic RNA-binding protein–RNA interactions.

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Fig. 1: The design of ARTR-seq.

References

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This is a summary of: Xiao Y. et al. Profiling of RNA-binding protein binding sites by in situ reverse transcription-based sequencing. Nat. Methods https://doi.org/10.1038/s41592-023-02146-w (2024).

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ARTR-seq for determining RNA-binding protein target sites through in situ reverse transcription. Nat Methods 21, 164–165 (2024). https://doi.org/10.1038/s41592-023-02147-9

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  • DOI: https://doi.org/10.1038/s41592-023-02147-9

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