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Engineered nanopores for exopeptidase protein sequencing

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Two studies show that nanopores can identify the 20 proteinogenic amino acids and some of their post-translational modifications. Coupled with an exopeptidase, a bottom-up approach to protein sequencing using nanopores is on the horizon.

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Fig. 1: Approaches for nanopore-based detection of peptide sequencing.

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Acknowledgements

A.B., A.S. and G.M. acknowledge NHGRI HG012554, NWO VICI # 10614 and the NW-XL21.003 # 14965 grants.

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Authors and Affiliations

  1. Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands

    Andrea Bonini, Adina Sauciuc & Giovanni Maglia

Authors
  1. Andrea Bonini
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  2. Adina Sauciuc
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  3. Giovanni Maglia
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Corresponding author

Correspondence to Giovanni Maglia.

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Competing interests

G.M. is a founder, director, and shareholder of Portal Biotech Limited, a company engaged in the development of nanopore technologies. A.S. and A.B. declare no competing interests

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Bonini, A., Sauciuc, A. & Maglia, G. Engineered nanopores for exopeptidase protein sequencing. Nat Methods 21, 16–17 (2024). https://doi.org/10.1038/s41592-023-02136-y

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